Osteonectin inhibiting de novo formation of apatite in the presence of collagen

Calcified Tissue International
Y DoiH Shimokawa

Abstract

The effect of bone matrix protein of osteonectin on de novo formation of apatite was studied in a wide range of calcium phosphate solutions in the presence of collagen. In every solution, from which amorphous calcium phosphate, octacalcium phosphate, or apatite precipitated as a possible initial phase, osteonectin at concentrations less than 1 microM retarded the precipitation, subsequent transformation to apatite, and ripening crystal growth of apatite. Collagen present as either reconstituted or denatured form had no effect on the osteonectin-associated reactions as well as osteonectin-free reactions, and no structural correlation was observed between collagen fibrils and any of the calcium phosphates that appeared in our system. Direct measurement of free calcium levels in the solutions suggested that the reduction in calcium activity due to complexing with osteonectin hardly explained the inhibitory activity of osteonectin in retarding the formation of apatite. Instead, our transmission electron microscopic (TEM) observation strongly suggested that the primary mechanism for osteonectin to inhibit the formation of apatite is to block growth sites of calcium phosphates nucleated. The apatite thus formed in the presence of ost...Continue Reading

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Citations

Aug 28, 2001·Journal of Biomedical Materials Research·Y LiuK de Groot
May 1, 1992·Calcified Tissue International·T ReichertL W Fisher
Feb 1, 1993·Calcified Tissue International·Y DoiH Shimokawa
Jun 1, 1992·Calcified Tissue International·F Bronner, W D Stein
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