PMID: 7536842Jan 1, 1995Paper

Osteopontin adhesion receptors on gingival fibroblasts

Journal of Periodontal Research
J D'ErricoM J Somerman

Abstract

Osteopontin (OPN) promotes attachment and spreading of cells in an RGD dependent fashion, suggesting that OPN interacts with integrins on cell surfaces. Here in, we show that LM-609, a monoclonal antibody to the alpha v beta 3 integrin (a vitronectin receptor), inhibited OPN-mediated attachment of gingival fibroblasts. To characterize the cell surface receptors responsible for this interaction, we performed OPN-sepharose affinity chromatography using detergent extracts of 35S-methionine or 125I-surface labeled gingival fibroblasts. Proteins bound to the OPN-matrix were eluted with EDTA and subjected to SDS-PAGE under reducing conditions. EDTA eluates from both 125I-surface labeled and 35S-methionine labeled extracts demonstrated prominent bands in the 90kDa and 50kDa regions, by both autoradiography and fluorography, respectively. These studies suggest that OPN is associated with other cell surface molecules in addition to alpha v beta 3. Furthermore, these as yet to be characterized proteins, may prove to have a stronger affinity for OPN than alpha v beta 3.

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Citations

Sep 22, 2012·International Journal of Oral Science·Brian L Foster
Jun 25, 2003·Journal of Neurotrauma·Masayuki HashimotoHideshige Moriya
Mar 24, 1999·Journal of Cellular Biochemistry·L L SmithC M Giachelli
Feb 1, 1997·Journal of Cellular Physiology·J Klein-NulendE H Burger
Dec 12, 2012·Journal of Oral Science·Janaina H JorgeEunice T Giampaolo

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