Osteopontin-deficient bone cells are defective in their ability to produce NO in response to pulsatile fluid flow

Biochemical and Biophysical Research Communications
D T DenhardtJ Klein-Nulend

Abstract

Osteopontin (OPN) is a noncollagenous component of bone matrix. It mediates cell attachment and activates signal transduction pathways. In this work, bone cells, cultured from fragments of long bones derived from wild-type and OPN-/- ("knock-out") mice, were exposed to pulsatile fluid flow (PFF) over a 60-min period. The medium was assayed periodically for nitric oxide (NO) and prostaglandin E(2) (PGE(2)) release. OPN+/+ cells exhibited a peak of NO production 5-10 min after the onset of PFF, decreasing to a stable plateau at 15 min; much less NO was produced by the OPN-/- cells. PFF resulted in reduced PGE(2) release by both cell types, although the reduction was less for the OPN-/- cells in the 15-30 min window. Both cell types exhibited a similar enhancement of cyclooxygenase2 mRNA levels 60 min after initiation of PFF. These results suggest that bone cells require OPN to respond fully to PFF as assessed by increased NO and reduced PGE(2) synthesis.

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Citations

Jun 28, 2008·Journal of Materials Science. Materials in Medicine·Elaine M ByrneBrian C O'Connell
Jun 15, 2011·The Journal of Cell Biology·Yayoi IzuDavid E Birk
Oct 24, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Noriaki MoriToshimitsu Uede
Jun 21, 2006·Ultrasound in Medicine & Biology·Diego Araujo Dalla-BonaKazuo Tanne
Mar 1, 2011·Biochimica Et Biophysica Acta·Margaret E WalkerMelissa A Brown
Jul 18, 2017·Antioxidants & Redox Signaling·David D RobertsJeffrey S Isenberg
Sep 29, 2017·PloS One·Wenping WangZhenxiang Wang
Jan 12, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Masahito KawaguchiNorio Kawahara

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