PMID: 7542900Apr 1, 1995Paper

Osteopontin gene expression and alkaline phosphatase activity in avian tibial dyschondroplasia

Bone
V KnopovM Pines

Abstract

Osteopontin (OPN) gene expression and alkaline phosphatase activity were evaluated in the epiphyseal growth plates of normal chickens and in diet-induced tibial dyschdroplasia (TD)-afflicted chickens. In the normal growth plate, OPN gene was expressed by a) cells of the subperichondrial zone surrounding the articular cartilage, b) a narrow layer of hypertrophic chondrocytes at the hypertrophic zone, and c) lower hypertrophic chondrocytes at the zone of matrix calcification and endochondral bone formation. The latter two layers were separated by OPN-negative chondrocytes. Osteopontin gene was not expressed throughout the zone of articular cartilage in the nonhypertrophic or upper hypertrophic portions of the growth plate cartilage. Only at sites of calcification of the lower hypertrophic zone was the expression of the OPN gene associated with alkaline phosphatase activity. In all TD lesions, regardless of the induction procedure, the layer of chondrocytes of the lower hypertrophic zone expressing the OPN gene and the layer of OPN-negative cells separating the two areas of OPN-expressing cells were grossly enlarged. This resulted in a wide discontinuity between the chondrocytes of the lower hypertrophic zone expressing the OPN ge...Continue Reading

Citations

Sep 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J WuR M Leach
Jun 22, 1999·Molecular and Cellular Endocrinology·S Ben-BassatM Pines
Sep 20, 2007·Poultry Science·R M Leach, E Monsonego-Ornan
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y WangD Givol
Nov 13, 2007·Avian Pathology : Journal of the W.V.P.A·C V GayR M Leach
Dec 1, 1995·Matrix Biology : Journal of the International Society for Matrix Biology·M PinesA Bar
Aug 5, 2006·Journal of Cellular Biochemistry·Eric P PourmandAdele L Boskey
Jan 1, 1996·Connective Tissue Research·A L Boskey

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