Osteoprotegerin (OPG) production by cells in the osteoblast lineage is regulated by pulsed electromagnetic fields in cultures grown on calcium phosphate substrates

Annals of Biomedical Engineering
Z SchwartzB D Boyan

Abstract

Pulsed electromagnetic fields (PEMF) used clinically to stimulate bone formation enhance the osteogenic effects of BMP-2 on human mesenchymal stem cells (MSCs) if the MSCs are grown in osteogenic medium and are cultured on calcium phosphate (CaP) surfaces rather than tissue culture polystyrene plastic (TCPS). This study tested if PEMF's effects on cells in the osteoblast lineage are substrate dependent and if factors produced by osteoblasts that regulate osteoclastic bone resorption, might also be regulated by PEMF. Human MSCs treated with BMP-2 and human osteoblast-like cells (normal human osteoblasts [NHOst cells], MG63 cells, SaOS-2 cells) were cultured on CaP or TCPS and their response to PEMF (4.5 ms bursts of 20 pulses repeating at 15 Hz for 8 h/day) determined as a function of decoy receptor osteoprotegerin (OPG) and RANK ligand (RANKL) production, both of which are associated with regulation of osteoclast differentiation. The results showed that when osteoblast-like cells were cultured on CaP, PEMF decreased cell number and increased production of paracrine factors associated with reduced bone resorption like OPG. RANKL was unaffected, indicating that the OPG/RANKL ratio was increased, further supporting a surface-depen...Continue Reading

References

Nov 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G Gronowicz, M B McCarthy
Mar 15, 2000·Journal of Orthopaedic Trauma·D C FredericksB Simon
Jun 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C H LohmannZ Schwartz
Oct 18, 2000·Journal of Biomedical Materials Research·E A CowlesG A Gronowicz
Oct 29, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C H LohmannB D Boyan
Mar 16, 2001·Clinical Orthopaedics and Related Research·H H GuerkovB D Boyan
Feb 9, 2002·Journal of Biomedical Materials Research·S R BannisterZ Schwartz
Mar 27, 2002·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·S LeisnerT Levin-Harrus
Oct 1, 2003·Journal of Biomedical Materials Research. Part a·Michele GilbertPatrick S Stayton
Mar 12, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Robyn A MacQuarrieGail I Anderson
Aug 10, 2004·Bioelectromagnetics·Walter Hong-Shong ChangFeng-Huei Lin
Sep 15, 2004·Biochimica Et Biophysica Acta·Y WittrantF Rédini
May 26, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Kyle ChangChung Shih
Jun 7, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ronald J MiduraAlan Wolfman
Feb 24, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Zvi SchwartzBarbara D Boyan
Aug 1, 2007·Journal of Materials Science. Materials in Medicine·M C SiebersJ A Jansen
Apr 9, 2008·Archives of Biochemistry and Biophysics·Brendan F Boyce, Lianping Xing
Apr 12, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Z SchwartzB D Boyan
May 10, 2008·Calcified Tissue International·S A J LloydTed A Bateman
Oct 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·R Olivares-NavarreteZ Schwartz

❮ Previous
Next ❯

Citations

Aug 21, 2010·Calcified Tissue International·Matthias WiensWerner E G Müller
May 24, 2011·Computer Methods in Biomechanics and Biomedical Engineering·Yanan Wang, Qing-Hua Qin
May 11, 2011·Journal of Dental Research·R A GittensZ Schwartz
Apr 9, 2011·Micron : the International Research and Review Journal for Microscopy·Berenice Noriega-LunaMario Avila-Rodriguez
Sep 3, 2013·Bioelectromagnetics·Rolando A GittensZvi Schwartz
Oct 6, 2012·Journal of Tissue Engineering and Regenerative Medicine·Emilia KaivosojaYrjö T Konttinen
Apr 19, 2016·Stem Cell Research & Therapy·Aleksandra MaziarzAgnieszka Banas
Feb 24, 2015·BioMed Research International·Karolina Sieroń-StołtnyAleksander Sieroń
Jun 30, 2016·Electromagnetic Biology and Medicine·Rong WangMingyu Song
Jun 30, 2016·Electromagnetic Biology and Medicine·Anna-Maria BiqueMervi Paulasto-Kröckel
Mar 17, 2018·Journal of the American Podiatric Medical Association·Kyle M L Unsdorfer, Samir M Abdelmagid
Feb 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francesca BalzanoMargherita Maioli
Sep 27, 2018·International Journal of Biomaterials·Carlo GalliStefano Guizzardi
Aug 18, 2021·International Journal of Radiation Biology·Neha AgrawalEkta Kohli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.