OsWOX3A is involved in negative feedback regulation of the gibberellic acid biosynthetic pathway in rice (Oryza sativa)

Journal of Experimental Botany
Sung-Hwan ChoNam-Chon Paek

Abstract

The plant-specific WUSCHEL-related homeobox (WOX) nuclear proteins have important roles in the transcriptional regulation of many developmental processes. Among the rice (Oryza sativa) WOX proteins, a loss of OsWOX3A function in narrow leaf2 (nal2) nal3 double mutants (termed nal2/3) causes pleiotropic effects, such as narrow and curly leaves, opened spikelets, narrow grains, more tillers, and fewer lateral roots, but almost normal plant height. To examine OsWOX3A function in more detail, transgenic rice overexpressing OsWOX3A (OsWOX3A-OX) were generated; unexpectedly, all of them consistently exhibited severe dwarfism with very short and wide leaves, a phenotype that resembles that of gibberellic acid (GA)-deficient or GA-insensitive mutants. Exogenous GA3 treatment fully rescued the developmental defects of OsWOX3A-OX plants, suggesting that constitutive overexpression of OsWOX3A downregulates GA biosynthesis. Quantitative analysis of GA intermediates revealed significantly reduced levels of GA20 and bioactive GA1 in OsWOX3A-OX, possibly due to downregulation of the expression of KAO, which encodes ent-kaurenoic acid oxidase, a GA biosynthetic enzyme. Yeast one-hybrid and electrophoretic mobility shift assays revealed that Os...Continue Reading

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Citations

May 14, 2016·Plant Signaling & Behavior·Sung-Hwan Cho, Nam-Chon Paek
Aug 17, 2017·International Journal of Molecular Sciences·Feng TangShihua Shen
Sep 7, 2017·Journal of Integrative Plant Biology·Changbin YinJiankang Wang
Nov 12, 2018·Journal of Integrative Plant Biology·Pan LiXin-Guang Zhu
Jan 31, 2019·Journal of Integrative Plant Biology·Sarah M McKim
Aug 31, 2019·International Journal of Molecular Sciences·Liaqat ShahChuanxi Ma
Mar 10, 2016·Journal of Experimental Botany·Million Tadege
Jul 12, 2020·Plant & Cell Physiology·Peter Hedden
Oct 24, 2020·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jiao WangYing Sun
Feb 17, 2021·Plant Physiology·Muhammad UzairXueyong Li
Jul 30, 2021·Frontiers in Plant Science·Ke ChenMariusz Jaremko

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