Otago glaucoma surgery outcome study: cytology and immunohistochemical staining of bleb capsules around Molteno implants

Investigative Ophthalmology & Visual Science
A C MoltenoAlex G Dempster

Abstract

To describe the cytology and immunohistochemistry of Molteno implant capsules from cases of primary and secondary glaucoma. Histologic features of capsules including cell cytology, the distribution of activated (proliferating) cells, apoptosing cells, and membrane bound vesicles (presumed death messengers) were assessed by light microscopy and correlated with clinicopathological features in 10 noninflamed eyes with good intraocular pressure control (nine autopsy and one enucleation) obtained from 2 months to 16.8 years after insertion of Molteno implants. All bleb capsules demonstrated two distinct layers. The thin external layer was cellular with fairly numerous small blood vessels coursing through normally staining, regularly arranged collagen fibers. The thicker, deeper layer was avascular, relatively acellular, and characterized by regularly arranged swollen and fragmented collagen fibers. Most cells in the external layer appeared normal; however, between 5% (in recently formed blebs) and approximately 50% (in well established blebs) showed cytological and/or immunohistochemical changes characteristic of metabolic activation and/or apoptosis. All cells in the deeper layer, regardless of time after surgery, also demonstrated...Continue Reading

Citations

Nov 26, 2015·Acta Ophthalmologica·Kyoung In Jung, Chan Kee Park
Jan 1, 2015·Journal of Current Glaucoma Practice·Joana FerreiraLuis Abegão Pinto

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