Ouabain Does Not Induce Selective Spiral Ganglion Cell Degeneration in Guinea Pigs

BioMed Research International
Timo SchomannMargriet A Huisman

Abstract

Round window membrane (RWM) application of ouabain is known to selectively destroy type I spiral ganglion cells (SGCs) in cochleas of several rodent species, while leaving hair cells intact. This protocol has been used in rats and Mongolian gerbils, but observations in the guinea pig are conflicting. This is why we reinvestigated the effect of ouabain on the guinea pig cochlea. Ouabain solutions of different concentrations were placed, in a piece of gelfoam, upon the RWM of the right cochleas. Auditory function was assessed using acoustically evoked auditory brainstem responses (aABR). Finally, cochleas were fixed and processed for histological examination. Due to variability within treatment groups, histological data was pooled and three categories based upon general histological observations were defined: cochleas without outer hair cell (OHC) and SGC loss (Category 1), cochleas with OHC loss only (Category 2), and cochleas with OHC and SGC loss (Category 3). Animals treated with 1 mM or 10 mM ouabain showed shifts in hearing thresholds, corresponding with varying histological changes in their cochleas. Most cochleas exhibited complete outer hair cell loss in the basal and middle turns, while some had no changes, together wit...Continue Reading

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Citations

Sep 10, 2021·Cell Transplantation·Tetsuji Sekiya, Matthew C Holley

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Methods Mentioned

BETA
cochlear implant

Software Mentioned

Leica Application Suite
ImageJ
MS Excel
GraphPad Prism
GraphPad
MATLAB

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