Ouabain Modulates Zymosan-Induced Peritonitis in Mice

Mediators of Inflammation
Jacqueline Alves LeiteSandra Rodrigues-Mascarenhas

Abstract

Ouabain, a potent inhibitor of the Na(+), K(+)-ATPase, was identified as an endogenous substance. Recently, ouabain was shown to affect various immunological processes. We have previously demonstrated the ability of ouabain to modulate inflammation, but little is known about the mechanisms involved. Thus, the aim of the present work is to evaluate the immune modulatory role of ouabain on zymosan-induced peritonitis in mice. Our results show that ouabain decreased plasma exudation (33%). After induction of inflammation, OUA treatment led to a 46% reduction in the total number of cells, as a reflex of a decrease of polymorphonuclear leukocytes, which does not appear to be due to cell death. Furthermore, OUA decreased TNF-α (57%) and IL-1β (58%) levels, without interfering with IL-6 and IL-10. Also, in vitro experiments show that ouabain did not affect endocytic capacity. Moreover, electrophoretic mobility shift assay (EMSA) shows that zymosan treatment increased (85%) NF-κB binding activity and that ouabain reduced (30%) NF-κB binding activity induced by zymosan. Therefore, our data suggest that ouabain modulated acute inflammatory response, reducing the number of cells and cytokines levels in the peritoneal cavity, as well as NF...Continue Reading

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Citations

Feb 26, 2016·Frontiers in Endocrinology·A M M OrellanaC Scavone
Dec 5, 2015·Frontiers in Physiology·Luiz Henrique Agra Cavalcante-SilvaSandra Rodrigues-Mascarenhas
Mar 7, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Tian ZhangTong Zhang
Sep 15, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·José G F M GalvãoSandra Rodrigues-Mascarenhas
Nov 28, 2017·Frontiers in Physiology·Luiz Henrique Agra Cavalcante-SilvaSandra Rodrigues-Mascarenhas
Jun 27, 2019·Journal of Immunology Research·Deyse C M CarvalhoSandra Rodrigues-Mascarenhas
Jun 12, 2019·Oxidative Medicine and Cellular Longevity·Chao ShaoJinjun Bian
May 16, 2019·Inflammopharmacology·Luiz Henrique Agra Cavalcante-SilvaSandra Rodrigues-Mascarenhas
Jul 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francesco MaioneStefano Pieretti

Methods Mentioned

BETA
lavage
Enzyme-Linked Immunosorbent Assay
Flow
FCS
flow cytometry
electrophoresis

Related Concepts

Metazoa
Cell Survival
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Macrophage
Ouabain
Secondary Peritonitis
Randomization
Zymosan
Supershift Mobility Assay

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