Out of the dark: transitional subsurface-to-surface microbial diversity in a terrestrial serpentinizing seep (Manleluag, Pangasinan, the Philippines)

Frontiers in Microbiology
Kristin M WoycheeseCarlo A Arcilla

Abstract

In the Zambales ophiolite range, terrestrial serpentinizing fluid seeps host diverse microbial assemblages. The fluids fall within the profile of Ca(2+)-OH(-)-type waters, indicative of active serpentinization, and are low in dissolved inorganic carbon (DIC) (<0.5 ppm). Influx of atmospheric carbon dioxide (CO2) affects the solubility of calcium carbonate as distance from the source increases, triggering the formation of meter-scale travertine terraces. Samples were collected at the source and along the outflow channel to determine subsurface microbial community response to surface exposure. DNA was extracted and submitted for high-throughput 16S rRNA gene sequencing on the Illumina MiSeq platform. Taxonomic assignment of the sequence data indicates that 8.1% of the total sequence reads at the source of the seep affiliate with the genus Methanobacterium. Other major classes detected at the source include anaerobic taxa such as Bacteroidetes (40.7% of total sequence reads) and Firmicutes (19.1% of total reads). Hydrogenophaga spp. increase in relative abundance as redox potential increases. At the carbonate terrace, 45% of sequence reads affiliate with Meiothermus spp. Taxonomic observations and geochemical data suggest that sev...Continue Reading

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Citations

Feb 26, 2015·Frontiers in Microbiology·Dawn CardaceCarlo A Arcilla
Feb 17, 2018·Extremophiles : Life Under Extreme Conditions·D'Arcy R Meyer-DombardCarlo A Arcilla
Jan 7, 2020·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·A S Templeton, E T Ellison
Apr 7, 2020·Environmental Microbiology·Mary C SabudaMatthew O Schrenk
Mar 16, 2019·The ISME Journal·Elizabeth M FonesEric S Boyd
Jun 8, 2017·Frontiers in Microbiology·Melitza Crespo-MedinaMatthew O Schrenk
Feb 23, 2017·Frontiers in Microbiology·Kaitlin R RempfertAlexis S Templeton

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Datasets Mentioned

BETA
PRJNA269301

Methods Mentioned

BETA
amplicon sequencing
X-ray
PCR

Software Mentioned

PyNAST
UCLUST
FastTree
QIIME

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