Outbreak of amoxicillin-resistant Haemophilus influenzae type b: variable number of tandem repeats as novel molecular markers.

Journal of Clinical Microbiology
A van BelkumJ F Meis

Abstract

An outbreak caused by amoxicillin-resistant Haemophilus influenzae type b was noted among patients suffering from chronic obstructive pulmonary disease. Since infections were clustered in time and place, an ongoing outbreak was suspected. The spread of the strain and the course of the outbreak could be followed by random amplification of polymorphic DNA (RAPD) analysis of the different bacterial isolates. In addition, studies were aimed at the determination of length polymorphism in regions of repetitive DNA. By PCR-mediated amplification of variable number of tandem repeat regions (VNTRs), additional insight into the genome composition of the epidemic strain was gained. Our results show that VNTRs comprising repeat units that are 3, 5, or 6 nucleotides in length provided stable genetic markers that can be used for molecular typing of H. influenzae type b. VNTRs built from tetranucleotide units, however, appear to be hypervariable and not suited for epidemiological studies. The observed variability in this latter class of VNTRs might be reminiscent of the bacterium's capacity to deal with unfavorable host factors.

References

Sep 1, 1991·The Journal of Hospital Infection·T A HekkerL van Alphen
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Oct 1, 1989·Journal of Clinical Microbiology·S TrottierC Svanborg-Edén
May 1, 1995·Journal of Clinical Microbiology·H C Smith-VaughanD J Kemp
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Aug 1, 1995·Journal of Clinical Microbiology·A SamuelsonA A Lindberg
Jun 1, 1995·Journal of Clinical Microbiology·A van BelkumH Koeleman
Jan 1, 1994·Current Biology : CB·E R MoxonR E Lenski
Sep 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J R KostmanJ J Plorde
Nov 1, 1993·Journal of Clinical Microbiology·J Z JordensM P Slack
Feb 22, 1996·Proceedings. Biological Sciences·D Field, C Wills
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W HoodE R Moxon
Nov 1, 1996·Journal of Clinical Microbiology·W van LeeuwenA van Belkum

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Citations

Sep 17, 2008·Mycoses·H A de ValkJ F G M Meis
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·N RendersA Van Belkum
Jun 29, 1999·Current Opinion in Microbiology·A van Belkum
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A van BelkumH Verbrugh
Oct 9, 2004·Journal of Clinical Microbiology·Ricardo Titze-de-AlmeidaAlex van Belkum

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