Outbreak of KPC-2-Producing Klebsiella pneumoniae ST76 Isolates in an Intensive Care Unit and Neurosurgery Unit.

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Shanshan SuXiaoli Zhang

Abstract

Background: The aim of this study was to investigate the characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP) ST76 isolates collected during an outbreak in a hospital's intensive care unit and neurosurgery unit. Methods: Seventeen separate clinical isolates of CRKP were collected from patients from March 2016 to February 2017. Bacterial isolates were identified, and antimicrobial susceptibility testing was conducted using the VITEK-2 compact system. Isolates containing antibiotic resistance genes were characterized by polymerase chain reaction and DNA sequencing. Clonal relatedness was assessed by multilocus sequence typing and pulsed-field gel electrophoresis. Conjugation experiments were performed to determine the transferability of plasmids with antibiotic resistance. The genomic features and mobile genetic elements of ST76 CRKP were detected by whole genome sequencing. Results: ST76 KPC-2-producing CRKP prevailed in our hospital, causing an outbreak. The strains also carried blaSHV-1, blaCTX-M-15, blaTEM-1, qnrB, and acc(6')Ib-cr resistance genes. Plasmids from 17.7% of the isolated strains bearing these resistance genes could be transferred into the recipient Escherichia coli J53 through conjugation. Sequen...Continue Reading

References

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Citations

Jul 24, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Wen-Xia ZhangJue Zhang
Aug 28, 2021·Antibiotics·Nadezhda K FursovaIvan A Dyatlov

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Glimmer
tRNAscan
SE
PlasmidFinder
BLASTn
ResFinder
BLAST
SOAPdenovo
Barrnap
Prokka

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