Outbreak of vancomycin-resistant Enterococcus faecium of the phenotype VanB in a hospital in Warsaw, Poland: probable transmission of the resistance determinants into an endemic vancomycin-susceptible strain

Journal of Clinical Microbiology
M KawalecW Hryniewicz

Abstract

The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infected and/or colonized by Enterococcus faecium that was resistant in vitro to vancomycin and susceptible to teicoplanin. PCR analysis confirmed that the vancomycin-resistant E. faecium (VREM) isolates carried the vanB gene, which is responsible for the VanB phenotype. Pulsed-field gel electrophoresis (PFGE) typing revealed that the isolates belonged to four distinct PFGE types and that one of these was clearly predominant, including isolates collected from seven different patients. The isolates contained one or more copies of the vanB gene cluster of the identical, unique DraI/PagI (BspHI) restriction fragment length polymorphism type, which resided in either the same or different plasmid molecules or chromosomal regions. All this data suggested that the outbreak was due to both clonal spread of a single strain and horizontal transfer of resistance genes among nonrelated strains, which could be mediated by plasmids and...Continue Reading

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Citations

Dec 24, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I KlareW Witte
Apr 6, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E SadowyW Hryniewicz
Jul 22, 2009·Antimicrobial Agents and Chemotherapy·Klaudia Kosowska-ShickPeter C Appelbaum
Aug 26, 2004·Antimicrobial Agents and Chemotherapy·Carla NovaisUNKNOWN Portuguese Resistance Study Group
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Carla NovaisUNKNOWN Portuguese Resistance Study Group
Nov 6, 2007·Journal of Clinical Microbiology·Janetta TopMarc Bonten
Apr 9, 2005·Journal of Clinical Microbiology·Lucia StamponeAnnalisa Pantosti
Nov 10, 2009·Future Microbiology·Rob Jl Willems, Willem van Schaik
May 30, 2009·Enfermedades infecciosas y microbiología clínica·Eva TorresGermán Bou
Jul 10, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M KawalecW Hryniewicz
Mar 30, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J TopM Bonten
Jul 9, 2008·Scandinavian Journal of Infectious Diseases·Balázs LibischRob J Willems

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