Outcome of bovine herpesvirus 4 infection following direct viral injection in the lateral ventricle of the mouse brain

Microbes and Infection
Gaetano DonofrioCarla Mucignat-Caretta

Abstract

A recombinant bovine herpesvirus 4 (BoHV-4EGFPDeltaTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the lateral ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPDeltaTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPDeltaTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in ...Continue Reading

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Citations

Feb 13, 2008·Genetic Vaccines and Therapy·Marco RedaelliGaetano Donofrio
Apr 23, 2014·PloS One·Valentina FranceschiGaetano Donofrio
Jul 6, 2014·Gene Therapy·A PuppoA Auricchio
Jun 19, 2015·PLoS Neglected Tropical Diseases·Valentina FranceschiGaetano Donofrio
Nov 10, 2017·Frontiers in Immunology·Andrea Elizabeth VernaGaetano Donofrio
Nov 25, 2016·Journal of Translational Medicine·Alfonso RosamiliaGaetano Donofrio

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