Outcomes of different concentrations of human amniotic fluid in a keratoconjunctivitis sicca-induced mouse model

International Ophthalmology
Guilherme G QuintoAshley Behrens

Abstract

To compare the effects of different concentrations of topical human amniotic fluid (HAF) in a mouse model of dry eye, forty C57BL/6 mice were divided into 4 treatment groups: 20 % HAF, 50 % HAF, 100 % HAF, and isotonic salt solution (control). Dry eye was induced by an injection of botulinum toxin B into the lacrimal gland. Tear production, ocular surface fluorescein staining, and blink rate were evaluated in each mouse at 5 time points during a 4-week period. Goblet cell density was assessed in stained histological sections. Regarding tear production, 20, 50, and 100 % HAF groups were all different from the control group (P < 0.001) at week 1. However, there were no statistically significant differences between the 20, 50, and 100 % HAF groups. At week 2, 20, 50, and 100 % HAF groups had significant improvement in staining score and were significantly different from the control group (P = 0.047, P = 0.005, and P = 0.001, respectively). No difference in spontaneous blink rate was observed between groups, at any time point. Goblet cell density was significantly decreased in the control group compared to the HAF treatment groups. All tested concentrations of topical HAF were effective and superior than the control in this keratoc...Continue Reading

References

Jan 1, 1973·International Ophthalmology Clinics·M A Lemp
Jun 1, 1995·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·M M al-QattanK Y Lin
Oct 20, 1998·Progress in Retinal and Eye Research·K Tsubota
Aug 6, 1999·The British Journal of Ophthalmology·K TsubotaS Shimmura
Feb 21, 2003·Journal of Neurosurgery·Güzin Yeşim Ozgenel, Gülaydan Filiz
Oct 19, 2004·The British Journal of Ophthalmology·G GeerlingD Hartwig
Mar 10, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·L LiuG Geerling
Jun 28, 2005·Investigative Ophthalmology & Visual Science·Shigeru NakamuraKazuo Tsubota
Jul 27, 2005·Investigative Ophthalmology & Visual Science·Stefano BarabinoM Reza Dana
Dec 31, 2005·Investigative Ophthalmology & Visual Science·Olan Suwan-apichonRoy S Chuck
Aug 1, 2006·American Journal of Ophthalmology·Samantha HerretesAshley Behrens
Nov 1, 2006·The Ocular Surface·Stephen C Pflugfelder
Nov 15, 2006·Cornea·Ashley BehrensUNKNOWN Dysfunctional tear syndrome study group
Jan 12, 2007·The Ocular Surface·Michael E Stern, Stephen C Pflugfelder
May 15, 2007·Molecular & Cellular Proteomics : MCP·Chan-Kyung J ChoEleftherios P Diamandis
Jun 19, 2007·Survey of Ophthalmology·Gary N Foulks
Jan 11, 2012·Archives of Ophthalmology·William StevensonReza Dana

❮ Previous
Next ❯

Citations

Dec 23, 2016·Cell Medicine·Maura K W BittencourtCristiane V Wenceslau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

Ophthalmic Plastic and Reconstructive Surgery
Yonca Ozkan Arat, Michael T Yen
Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
Kaevalin LekhanontRoy S Chuck
© 2022 Meta ULC. All rights reserved