Outer-capsid P8 proteins of phytoreoviruses mediate secretion of assembled virus-like particles from insect cells

The Journal of General Virology
Naoyuki MiyazakiToshihiro Omura

Abstract

Phytoreoviruses are composed of two concentric capsid layers that surround a viral genome. The capsids are formed mainly by the inner-capsid P3 protein and the outer-capsid P8 protein. During the infection of insect-vector cells, these play important roles in packaging the viral genome and the enzymes required for its transcription. P3 and P8 proteins, when co-expressed in Spodoptera frugiperda cells, co-localized in cells and were released as spherical clusters. In contrast P3 proteins expressed in the absence of P8 protein were associated with the cells when they were examined by confocal microscopy. Cryo-electron microscopy revealed that the secreted clusters, composed of P3 and P8 proteins, were double-layered virus-like particles that were indistinguishable from intact viral particles. Our results indicate that P8 proteins mediate the secretion of assembled virus-like particles from S. frugiperda insect cells and, therefore, most probably from insect-vector cells also.

References

Nov 5, 1999·Advances in Virus Research·T Omura, J Yan
Oct 31, 2007·Journal of Electron Microscopy·Sumie KatayamaKenji Iwasaki
Sep 13, 2008·Journal of Virology·Naoyuki MiyazakiR Holland Cheng
Oct 22, 2008·The Journal of General Virology·Taiyun WeiToshihiro Omura
Apr 17, 2009·Journal of Virology·Cristina C P Celma, Polly Roy
Mar 2, 2010·Journal of Biochemistry·Naoyuki MiyazakiR Holland Cheng

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Citations

Oct 15, 2013·Journal of Synchrotron Radiation·Naoyuki MiyazakiKenji Iwasaki

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