PMID: 11319151Apr 25, 2001Paper

Outer dense fiber proteins are dominant postobstruction autoantigens in adult Lewis rats

Biology of Reproduction
C J FlickingerJ C Herr

Abstract

Obstruction of the male reproductive tract commonly results in generation of antisperm autoantibodies. However, only a few of the sperm autoantigens recognized by these antibodies have been characterized. To identify postobstruction rat sperm autoantigens, sperm proteins were separated by two-dimensional(2-D) gel electrophoresis. Spots corresponding to proteins that were stained by at least 50% of postvasectomy rat sera on 2-D Western blots were removed from polyacrylamide gels and microsequenced by tandem mass spectrometry. From a total of 21 spots, 12 contained peptides that matched solely to either of two outer dense fiber proteins, odf1 or odf2. Six additional spots contained peptides comprising odf1 or odf2 and were accompanied by peptides representing other proteins. Only three spots lacked outer dense fiber peptides but did contain sequences of other known proteins. The results indicate that the outer dense fiber proteins odf1 and odf2 are dominant postobstruction autoantigens because they were detected in the majority of the immunoreactive protein spots examined. Possible explanations for this observation include the abundance of outer dense fiber proteins in spermatozoa, slow solubility, which may provide a sustained s...Continue Reading

References

Sep 1, 1979·Fertility and Sterility·N J Alexander, D J Anderson
Jun 1, 1975·Developmental Biology·D W Fawcett
Nov 1, 1990·Developmental Biology·F A van der HoornS K Nordeen
Dec 2, 1991·Biochimica Et Biophysica Acta·T MoritaA Matsushiro
Sep 1, 1990·Biology of Reproduction·J M BaltzR A Cone
Dec 1, 1986·The Anatomical Record·S P FlahertyG E Olson
Feb 1, 1989·Biology of Reproduction·J C HerrC J Flickinger
Jul 1, 1989·Biology of Reproduction·R K NazH Fisch
Sep 1, 1988·Analytical Biochemistry·D F HochstrasserC R Merril
Dec 1, 1988·Biology of Reproduction·H H HandleyJ C Herr
Jan 1, 1985·Journal of Reproductive Immunology·S Naaby-Hansen, O J Bjerrum
Jun 1, 1974·Experimental Cell Research·H I Calvin, G Bleau
Jun 1, 1983·American Journal of Reproductive Immunology : AJRI·C Y LeeY S Huang
Feb 1, 1982·The Anatomical Record·C J Flickinger
Apr 1, 1982·The Anatomical Record·M J Irons, Y Clermont
Mar 1, 1980·Biology of Reproduction·G E Olson, D W Sammons
Nov 1, 1980·The Anatomical Record·S W Kennedy, P M Heidger
Oct 20, 1995·The Journal of Biological Chemistry·M M MaxwellN Aziz
Feb 1, 1995·Journal of Reproductive Immunology·C J FlickingerJ C Herr
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Apr 11, 1997·The Journal of Biological Chemistry·H BrohmannS Hoyer-Fender
Mar 2, 1999·Reviews of Reproduction·E M Eddy
Jul 20, 1999·Biology of Reproduction·J C HerrC J Flickinger

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Citations

May 2, 2002·Molecular Reproduction and Development·Leigh Ann BushCharles J Flickinger
Jan 10, 2004·Antioxidants & Redox Signaling·Antonio Miranda-VizueteRichard Oko
Dec 8, 2009·Journal of Reproductive Immunology·Soren Naaby-Hansen, John C Herr
Aug 11, 2005·The Journal of Pathology·Monika FijakAndreas Meinhardt
Jan 24, 2007·Biochemical and Biophysical Research Communications·Monali Wakle, Vrinda Khole
Mar 21, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Bharati G KhobarekarAtmaram H Bandivdekar
Jul 6, 2004·Molecular Reproduction and Development·Jana AuerMarta De Almeida
Sep 26, 2006·Molecular Reproduction and Development·Satish Bhande, Rajesh K Naz
Apr 26, 2006·American Journal of Reproductive Immunology : AJRI·Agnieszka ParadowskaWalter Krause
Jan 11, 2020·Free Radical Biology & Medicine·Kaushiki KadamBini Ramachandran

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