Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.

Probiotics and Antimicrobial Proteins
Huan WangYuanhong Xie

Abstract

Plantaricin BM-1, a class IIa bacteriocin produced by Lactobacillus plantarum BM-1, has significant antibacterial activity against Gram-positive and Gram-negative bacteria. This study aimed to explore the role of the Escherichia coli K12 outer membrane (OM) channel protein TolC in the response to plantaricin BM-1. The tolC null mutant (E. coli K12∆tolC) was constructed by Red homologous recombination. The mechanism of tolC regulating the sensitivity of E. coli K12 under plantaricin BM-1 was investigated. tolC null mutation significantly increased the E. coli K12 sensitivity to plantaricin BM-1 and inhibited biofilm formation, and cells ruptured and shrunk. Proteomic analysis showed that the AcrAB-TolC and EmrAB-TolC efflux pumps were significantly (p < 0.05) upregulated in E. coli K12∆tolC. Based on the results of real-time PCR, we concluded that under plantaricin BM-1, the CpxR/CpxA two-component regulatory system of E. coli K12 responded with envelope damage, followed by activation of the transcription of marA and expression of AcrAB-TolC efflux pump. Moreover, tolC null mutation weakened the AcrAB-TolC efflux pump and then increased the sensitivity of E. coli K12 to plantaricin BM-1. These will contribute exploring the actio...Continue Reading

References

Sep 1, 1993·FEMS Microbiology Reviews·T R Klaenhammer
Oct 1, 1996·Journal of Bacteriology·H Nikaido
Jul 23, 1998·Journal of Applied Microbiology·E HubertC Padilla
Jan 5, 2002·International Journal of Food Microbiology·J ClevelandM L Chikindas
Jul 27, 2002·Annual Review of Microbiology·Margaret A Riley, John E Wertz
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Djamel DriderHervé Prévost
Apr 15, 2008·Molecular Cell·Vassiliy N BavroBen Luisi
Oct 2, 2008·Applied Microbiology and Biotechnology·Natthawut WiriyathanawudhiwongHiroshi Takagi
Dec 27, 2012·Nature Reviews. Microbiology·Paul D CotterColin Hill
Aug 28, 2013·Antimicrobial Agents and Chemotherapy·Ken-ichi OkudaYoshimitsu Mizunoe
Aug 21, 2014·World Journal of Microbiology & Biotechnology·Gargi Pal, Sheela Srivastava
Dec 30, 2014·Frontiers in Microbiology·Shih-Chun YangJia-You Fang
May 1, 2015·Antibiotics·Victor I Band, David S Weiss
Aug 19, 2016·Biochemistry·Stanislav D ZakharovWilliam A Cramer

❮ Previous
Next ❯

Citations

Oct 16, 2021·Biotechnology Letters·Gulyaim K AbitayevaSerik M Shaikhin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.