Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides.

Infection and Immunity
J A BengoecheaI Moriyón

Abstract

Sensitivities to polycationic peptides and EDTA were compared in Yersinia enterocolitica pathogenic and environmental biogroups. As shown by changes in permeability to the fluorescent hydrophobic probe N-phenylnaphthylamine (NPN), the outer membranes (OMs) of pathogenic and environmental strains grown at 26 degrees C in standard broth were more resistant to poly-L-lysine, poly-L-ornithine, melittin, cecropin P1, polymyxin B, and EDTA than Escherichia coli OMs. At 37 degrees C, OMs of pathogenic biogroups were resistant to EDTA and polycations and OMs of environmental strains were resistant to EDTA whereas E. coli OMs were sensitive to both EDTA and polycations. Similar results were found when testing deoxycholate sensitivity after polycation exposure or when isogenic pairs with or without virulence plasmid pYV were compared. With bacteria grown without Ca++ available, OM permeability to NPN was drastically increased in pathogenic but not in environmental strains or E. coli. Under these conditions, OMs of pYV+ and pYV- cells showed small differences in NPN permeability but differences in polycation sensitivity could not be detected by fluorimetry. O:1,6 (environmental type) lipopolysaccharide (LPS), but not O:3 or O:8 LPS, was m...Continue Reading

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Citations

Apr 21, 2005·Extremophiles : Life Under Extreme Conditions·Arantxa PeñaRamon Rosselló-Mora
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Miguel A CamposJosé A Bengoechea
Mar 29, 2000·Journal of Applied Microbiology·I M Helander, T Mattila-Sandholm
Nov 24, 2004·Infection and Immunity·Miguel A CamposJosé A Bengoechea
Mar 17, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Birute PudziuvyteGiulio Jori
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Enrique LlobetJosé A Bengoechea
May 1, 2015·Antibiotics·Victor I Band, David S Weiss
Jun 17, 2003·Journal of Applied Microbiology·S LangsrudG Sundheim
Apr 17, 2012·Journal of Bacteriology·Mar ReinésJosé A Bengoechea
Feb 13, 2019·Infection and Immunity·Toby L BartholomewJosé A Bengoechea
Mar 17, 2010·International Journal of Antimicrobial Agents·R RasulM D P Willcox

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