Outer membrane lipoprotein RlpA is a novel periplasmic interaction partner of the cell division protein FtsK in Escherichia coli

Scientific Reports
Alison M BerezukCezar M Khursigara

Abstract

In Escherichia coli, formation of new cells is mediated by the elongasome and divisome that govern cell elongation and septation, respectively. Proper transition between these events is essential to ensure viable progeny are produced; however, the components of each complex responsible for transmission of the cell signal to shift from elongation to septation are unclear. Recently, a region within the N-terminal domain of the essential divisome protein FtsK (FtsKN) was identified that points to a key role for FtsK as a checkpoint of cell envelope remodeling during division. Here, we used site-specific in vivo UV cross-linking to probe the periplasmic loops of FtsKN for protein interaction partners critical for FtsKN function. Mass spectrometry analysis of five unique FtsKN periplasmic cross-links revealed a network of potential FtsKN interactors, one of which included the septal peptidoglycan binding protein rare lipoprotein A (RlpA). This protein was further verified as a novel interaction partner of FtsKN by an in vitro pull-down assay. Deletion of rlpA from an FtsK temperature-sensitive E. coli strain partially restored cell growth and largely suppressed cellular filamentation compared to the wild-type strain. This suggests t...Continue Reading

References

Apr 1, 1991·Molecular Microbiology·N Nanninga
Nov 1, 1995·Journal of Bacteriology·K J BeggW D Donachie
May 17, 1994·Biochemistry·G Dormán, G D Prestwich
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A MukherjeeJ Lutkenhaus
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J PoglianoJ Beckwith
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M von RechenbergJ V Höltje
Aug 29, 1998·Journal of Bacteriology·G C DraperW D Donachie
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Sep 7, 2001·Nature·F van den EntJ Löwe
Aug 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jason W ChinPeter G Schultz
Oct 31, 2002·Chembiochem : a European Journal of Chemical Biology·Jason W Chin, Peter G Schultz
Feb 4, 2003·Journal of Bacteriology·Lawrence Rothfield
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Jul 12, 2005·Current Biology : CB·Nathan W Goehring, Jon Beckwith
Jun 8, 2006·Annual Review of Biochemistry·Katharine A Michie, Jan Löwe
Jul 15, 2006·The Journal of Biological Chemistry·Petra BornWaldemar Vollmer
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hiroyuki Mori, Koreaki Ito
May 22, 2007·Molecular Microbiology·Sarah BigotFrançois Cornet
Nov 2, 2007·Molecular Microbiology·François-Xavier Barre
Feb 4, 2010·Expert Review of Proteomics·Deborah H LundgrenDavid K Han
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Thomas J SilhavySuzanne Walker
Sep 10, 2010·Annual Review of Microbiology·Kevin D Young
Nov 26, 2010·Molecular Microbiology·Nelly DubarryFrançois-Xavier Barre
Dec 16, 2010·Molecular Microbiology·Ian Grainge
Feb 18, 2011·Gut Microbes·Javier López-Garrido, Josep Casadesús
Dec 29, 2011·Nature Reviews. Microbiology·Athanasios TypasWaldemar Vollmer
Nov 20, 2012·Advances in Experimental Medicine and Biology·Gaëlle DemarreFrançois-Xavier Barre

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Citations

Dec 6, 2019·Nature Communications·Martín AlcorloJuan A Hermoso
Jan 24, 2020·Canadian Journal of Microbiology·Alison M BerezukCezar M Khursigara
Feb 25, 2020·Annual Review of Biophysics·Ryan McQuillen, Jie Xiao
Jun 19, 2019·Current Genetics·Mingzhi WangZheng Hou
Oct 8, 2021·Frontiers in Microbiology·Isis Gabriela Barbosa CarvalhoAlessandra Alves de Souza

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Methods Mentioned

BETA
pull-down
two-hybrid
protein assay
PCR

Software Mentioned

Scaffold
STRING
PEAKS
Prism
Proteome
ImageJ

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