Abstract
The outer membrane proteins of Actinobacillus pleuropneumoniae are mediators of infection, acting as targets for the host's defense system. The outer membrane lipoprotein VacJ is involved in serum resistance and intercellular spreading in several pathogenic bacteria. To investigate the role of VacJ in the pathogenicity of Actinobacillus pleuropneumoniae, the vacJ gene-deletion mutant MD12 ΔvacJ was constructed. The increased susceptibility to KCl, SDS plus EDTA, and several antibiotics in the MD12ΔvacJ mutant suggested that the stability of the outer membrane was impaired as a result of the mutation in the vacJ gene. The increased NPN fluorescence and significant cellular morphological variation in the MD12ΔvacJ mutant further demonstrated the crucial role of the VacJ lipoprotein in maintaining the outer membrane integrity of A. pleuropneumoniae. In addition, the MD12ΔvacJ mutant exhibited decreased survival from the serum and complement killing compared to the wild-type strain. Interestingly, the MD12ΔvacJ mutant showed reduced biofilm formation compared to the wild-type strain. To our knowledge, this is the first description of the VacJ lipoprotein contributing to bacterial biofilm formation. The data presented in this study ...Continue Reading
References
Jul 4, 1995·Gene·S E WestR A Welch
Jan 1, 1994·Molecular Microbiology·T SuzukiC Sasakawa
Feb 28, 1998·Nucleic Acids Research·J D ThompsonD G Higgins
Sep 11, 1999·FEMS Microbiology Letters·W OswaldG Gerlach
Mar 7, 2002·Microbes and Infection·Janine T BosséPaul R Langford
Mar 20, 2003·FEMS Microbiology Letters·Nina BaltesGerald F Gerlach
Jul 24, 2003·Protein Science : a Publication of the Protein Society·Agnieszka S JunckerAnders Krogh
May 27, 2005·Veterinary Microbiology·Jeffrey B Kaplan, Martha H Mulks
Apr 6, 2006·Journal of Bacteriology·M Madan BabuK Sankaran
May 4, 2007·Proteomics·Jacqueline W ChungJames W Coulton
Mar 4, 2008·Microbiology·Claire LewisMark Roberts
Sep 23, 2008·Journal of Bacteriology·Marie-Stéphanie AschtgenEric Cascales
Oct 27, 2010·Infection and Immunity·A Kovacs-SimonS L Michell
Jan 22, 2011·PLoS Pathogens·Shigeki NakamuraJeffery N Weiser
Mar 28, 2012·Infection and Immunity·Deborah L BaldiMarija Tauschek
Jan 18, 2013·PloS One·Fang XieChunlai Wang
Aug 6, 2013·The Journal of Infectious Diseases·Pei-Fang HsiehJin-Town Wang
Jan 31, 2014·Infection and Immunity·Chandra D CarpenterShelley M Payne
Apr 16, 2014·Research in Veterinary Science·Sathish Bhadravati ShivachandraMuthannan Andavar Ramakrishnan
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Oct 11, 2014·Nucleic Acids Research·Ivica LetunicPeer Bork
Citations
Aug 12, 2016·PloS One·Xiaojun WuJason F Huntley
Nov 1, 2016·Journal of Proteome Research·Karl MummRita Hõrak
Dec 19, 2016·Gene·Liangyou ZhaoShimin Zheng
Nov 8, 2017·Animal Health Research Reviews·Skander HathroubiMario Jacques
Nov 11, 2017·Veterinary Research·Fabio AntenucciAnders Miki Bojesen
Jul 11, 2018·Journal of Bacteriology·Steve P BernierMichael G Surette
Mar 11, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·William Gustavo LimaMagna Cristina Paiva
May 4, 2018·Scientific Reports·Ariadna Fernández-CalvetJunkal Garmendia
Jan 11, 2018·Veterinary Research·Fabio AntenucciAnders Miki Bojesen
Feb 6, 2020·Journal of Bacteriology·Gang LiSiguo Liu
Jun 12, 2018·Frontiers in Microbiology·Mo-Fei Li, Li Sun
Jul 19, 2018·Frontiers in Microbiology·Jinlin LiuChao Qi
May 26, 2017·Frontiers in Microbiology·Fang XieChunlai Wang
Aug 14, 2018·Frontiers in Microbiology·Jia YanLiang Ma
May 29, 2020·BMC Veterinary Research·Samantha J HauUNKNOWN BRaDP1T Consortium
Jul 10, 2020·AMB Express·Jiameng XiaoLiancheng Lei
Oct 17, 2020·Molecular Oral Microbiology·Ok-Jin ParkSeung Hyun Han
Apr 5, 2021·Metabolic Engineering·Miguel C Santoscoy, Laura R Jarboe