Outer membrane protein A of Escherichia coli O157:H7 stimulates dendritic cell activation.

Infection and Immunity
Alfredo G TorresLynn Soong

Abstract

Outer membrane protein A (OmpA) is located in the membrane of Escherichia coli and other gram-negative bacteria and plays a multifunctional role in bacterial physiology and pathogenesis. In enterohemorrhagic E. coli (EHEC), especially serotype O157:H7, OmpA interacts with cultured human intestinal cells and likely acts as an important component to stimulate the immune response during infection. To test this hypothesis, we analyzed the effect of EHEC OmpA on cytokine production by dendritic cells (DCs) and on DC migration across polarized intestinal epithelial cells. OmpA induced murine DCs to secrete interleukin-1 (IL-1), IL-10, and IL-12 in a dose-dependent manner, and this effect was independent of Toll-like receptor 4. Although DCs displayed differential responses to EHEC OmpA and OmpA-specific antibodies enhanced DC cytokine secretion, we cannot discard that other EHEC surface elements were likely to be involved. While OmpA was required for bacterial binding to polarized Caco-2 cells, it was not needed for the induction of cytokine production by Caco-2 cells or for human DC migration across polarized cells.

References

Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Jan 10, 1980·The New England Journal of Medicine·M H Ulshen, J L Rollo
Jan 1, 1996·Advances in Experimental Medicine and Biology·J T Poolman
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Jul 17, 1998·Clinical Microbiology Reviews·J C Paton, A W Paton
Oct 26, 1999·Infection and Immunity·C M ThorpeD W Acheson
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C SoulasP Jeannin
Aug 30, 2000·Nature·A Aderem, R J Ulevitch
Nov 1, 2000·Epidemiology and Infection·S MakinoT Tsukamoto
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·F NiedergangJ P Kraehenbuhl
Jan 13, 2001·Springer Seminars in Immunopathology·C CauxA Vicari
Mar 20, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A IzadpanahM F Kagnoff
Jun 8, 2001·Trends in Microbiology·R S HeydermanT R Hirst
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H QiL Soong
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·F SierroJ C Sirard
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T D Eaves-PylesR B Pyles
Jan 5, 2002·Microbes and Infection·L Eckmann, M F Kagnoff
Jan 10, 2002·The Journal of Experimental Medicine·Sebastian Amigorena
Jan 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christine SedlikSebastian Amigorena
Jul 17, 2003·Cellular Microbiology·Heidrun Moll
Aug 23, 2003·Infection and Immunity·Alfredo G Torres, James B Kaper
Mar 24, 2004·Trends in Microbiology·Florence NiedergangJean-Claude Sirard
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jaime A TobarAlexis M Kalergis
Dec 25, 2004·Infection and Immunity·Alfredo G TorresJames B Kaper
Apr 13, 2005·Biochemical and Biophysical Research Communications·Sooan ShinKwang-Sik Kim

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Citations

Oct 3, 2009·Infection and Immunity·Tracy F NicholsonDavid A Hunstad
Jul 17, 2012·Journal of Bacteriology·Verónica I Martínez-SantosJosé L Puente
Jul 17, 2007·PLoS Pathogens·Paula RistowMathieu Picardeau
Sep 19, 2012·Veterinary Microbiology·Anthony W Confer, Sahlu Ayalew
Jan 14, 2012·The FEBS Journal·Subramanian Krishnan, Nemani V Prasadarao
Aug 22, 2009·Biochimica Et Biophysica Acta·Sang-Hyun KimKyu-Tae Chang
Jun 11, 2015·Future Microbiology·Javier Sanchez-Villamil, Fernando Navarro-Garcia
May 5, 2012·Gut Microbes·Abigail ClementsGad Frankel
Jun 15, 2007·FEMS Microbiology Letters·Stephen G J SmithRobert P Fagan
Feb 9, 2012·Memórias do Instituto Oswaldo Cruz·Ana Carolina Ramos MorenoMarina Baquerizo Martinez
Sep 6, 2013·Microbiology and Molecular Biology Reviews : MMBR·Vanessa Liévin-Le Moal, Alain L Servin
May 22, 2009·Journal of Neuropathology and Experimental Neurology·Hsueh-Hsia WuMei-Ru Chen
Nov 3, 2007·Microbiology·Eric A Brown, Philip R Hardwidge
May 5, 2017·Frontiers in Cellular and Infection Microbiology·Tungadri BoseSharmila S Mande
Jun 25, 2015·Microbiology Spectrum·Brian D McWilliams, Alfredo G Torres
Jun 25, 2015·Microbiology Spectrum·Jay L Mellies, Emily Lorenzen
Jul 3, 2021·Toxins·Kyung-Soo LeeMoo-Seung Lee

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