Outer membrane protein DsrA is the major fibronectin-binding determinant of Haemophilus ducreyi.

Infection and Immunity
Isabelle LeducChristopher Elkins

Abstract

The ability to bind extracellular matrix proteins is a critical virulence determinant for skin pathogens. Haemophilus ducreyi, the etiological agent of the genital ulcer disease chancroid, binds extracellular matrix components, including fibronectin (FN). We investigated H. ducreyi FN binding and report several important findings about this interaction. First, FN binding by H. ducreyi was greatly increased in bacteria grown on heme and almost completely inhibited by hemoglobin. Second, wild-type strain 35000HP bound significantly more FN than did a dsrA mutant in two different FN binding assays. Third, the expression of dsrA in the dsrA mutant restored FN binding and conferred the ability to bind FN to a non-FN-binding Haemophilus influenzae strain. Fourth, an anti-DsrA monoclonal antibody partially blocked FN binding by H. ducreyi. The hemoglobin receptor, the collagen-binding protein, the H. ducreyi lectin, the fine-tangle pili, and the outer membrane protein OmpA2 were not involved in H. ducreyi FN binding, since single mutants bound FN as well as the parent strain did. However, the major outer membrane protein may have a minor role in FN binding by H. ducreyi, since a double dsrA momp mutant bound less FN than did the singl...Continue Reading

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Citations

Nov 18, 2009·Current Opinion in Infectious Diseases·Diane M JanowiczMargaret E Bauer
Nov 5, 2008·Infection and Immunity·Dagmara I Kisiela, Charles J Czuprynski
Mar 24, 2005·Infection and Immunity·Catherine Dinitra WhiteChristopher Elkins
Dec 24, 2008·Journal of Bacteriology·Jeremiah J Davie, Anthony A Campagnari
Feb 18, 2009·Microbial Cell Factories·Cristiana CastaldoMargherita Sacco
Aug 11, 2010·FEMS Microbiology Reviews·Brian HendersonMark A Williams
Apr 22, 2015·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·William G FuscoIsabelle Leduc
Sep 18, 2013·Infection and Immunity·William G FuscoIsabelle Leduc
Jun 20, 2019·Frontiers in Microbiology·Ina MeuskensDirk Linke
Jun 14, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jacob C GrantStanley M Spinola

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