Outer membrane protein of Neisseria meningitidis as a mucosal adjuvant for lipopolysaccharide of Brucella melitensis in mouse and guinea pig intranasal immunization models.

Infection and Immunity
L L Van De VergR L Warren

Abstract

A mucosal vaccine against brucellosis consisting of the lipopolysaccharide (LPS) of Brucella melitensis complexed with the outer membrane protein (GBOMP) of group B Neisseria meningitidis was tested in small-animal models of intranasal immunization. Mice given two doses of the vaccine developed high levels of immunoglobulin G (IgG) and IgA antibodies specific for B. melitensis LPS in lung lavages and specific IgG and IgA antibody-secreting cells in the lungs and spleen. Similarly, in guinea pigs immunized twice intranasally, IgG and IgA LPS-specific antibodies were detected in lung lavages, and specific antibody-secreting cells were isolated from the spleen and cervical nodes. In mice immunized with LPS only, pulmonary responses consisted mostly of IgM antibodies, while guinea pigs given LPS alone developed local antibody of all three isotypes, but at lower levels compared to animals given the complex vaccine. Both mice and guinea pigs also developed high levels of serum IgG and moderate levels of IgA as a result of intranasal immunization with the complex vaccine. The serum antibodies in both cases were found to cross-react with the LPS of B. abortus, which shares an immunogenic epitope with B. melitensis LPS. In mice given th...Continue Reading

References

May 1, 1979·The Journal of Clinical Investigation·W D ZollingerS Berman
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M A LiuL A Hawe
Jan 1, 1987·Annales De L'Institut Pasteur. Microbiology·D R BundleM B Perry
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Nov 1, 1972·Infection and Immunity·W D ZollingerM S Artenstein
Dec 16, 1983·Journal of Immunological Methods·C C CzerkinskyA Tarkowski
May 31, 1995·Annals of the New York Academy of Sciences·C R Alving
Nov 1, 1993·Infection and Immunity·Y Zhan, C Cheers
Jul 1, 1964·The Journal of Experimental Medicine·G B MACKANESS

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Citations

Jul 6, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·T J CieslakE M Eitzen
Aug 19, 2000·Infection and Immunity·M J IzadjooD L Hoover
Mar 30, 2001·American Journal of Veterinary Research·M G MenseD L Hoover
Apr 28, 2010·Vector Borne and Zoonotic Diseases·Sung-Il LeeIbulaimu Kakoma
Aug 9, 2007·Expert Opinion on Drug Delivery·Dmytro S SilinAldar S Bourinbaiar

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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.