Outer membrane protein U (OmpU) mediates adhesion of Vibrio mimicus to host cells via two novel N-terminal motifs

PloS One
Xueqin LiuLin Li

Abstract

Vibrio mimicus (V.mimicus) is a causative agent of ascites disease in aquatic animals. Our previous studies have demonstrated that the outer membrane protein U (OmpU) from V.mimicus is an immunoprotective antigen with six immunodominant linear B-cell epitopes. Although the N-terminus of OmpU contains potential binding motifs, it remained unclear whether OmpU possesses adhesion function. Here, the adhesive capacity of recombinant OmpU and V.mimicus to epithelioma papulosum cyprinid (EPC) cells was determined by immunofluorescence and adherence assay. The results showed that after co-incubated with rOmpU, an obvious visible green fluorescence could be observed on the EPC cell surface and the nuclei exhibited blue fluorescence; while the control cell surface did not show any signal, only nuclei exhibited blue fluorescence. The average number of wild-type strain adhered to each cell was 32.3 ± 4.5. The average adhesion number of OmpU gene deletion mutant was significantly reduced to 10.8 ± 0.5 (P < 0.01) and restored to 31.3 ± 2.8 by complement strain (P >0.05). Pretreatment of cells with rOmpU reduced the average adhesion number of wild-type strain to 9.7 ± 2.9 (P < 0.01). Likewise, binding was significantly decreased to 8.8 ± 3.2...Continue Reading

References

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Citations

Jul 15, 2015·World Journal of Microbiology & Biotechnology·Rui LinWenxiong Lin
May 13, 2018·World Journal of Microbiology & Biotechnology·George Osei-AdjeiYiquan Zhang
May 11, 2016·Journal of Basic Microbiology·El-Shaymaa Abdel-SattarAbdelaziz Elgaml
Nov 30, 2018·Pathogens·Sophie RodriguesAlexis Bazire
Jan 27, 2018·Acta Crystallographica. Section D, Structural Biology·Huanyu LiChangjiang Dong

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Methods Mentioned

BETA
PCR
electrophoresis
Protein Assay
FCS
flow cytometry

Software Mentioned

SAS

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