Outer membrane proteins and DNA profiles in strains of Haemophilus parasuis recovered from systemic and respiratory sites

Journal of Clinical Microbiology
A RuizC Pijoan

Abstract

Polyserositis caused by Haemophilus parasuis is an important disease that affects mostly weaned pigs. Recent studies have shown that virulence can differ among strains recovered from distinct body sites and also that it may be related to the presence of certain outer membrane proteins (OMPs). The objective of this study was to compare the OMP and DNA profiles of H. parasuis strains isolated from systemic and respiratory sites from diseased and healthy pigs. Strains evaluated in this study were processed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and repetitive-PCR techniques. Two experiments were conducted in order to better define the relationship among genotype, phenotype, and site of isolation. Experiment 1 included 53 H. parasuis isolates recovered from healthy and diseased pigs from unrelated herds. Experiment 2 included 31 isolates of H. parasuis obtained from diseased pigs involved in an outbreak in a large, multifarm system. Results showed that strains recovered from systemic sites had more homogeneous OMP and DNA profiles than those isolated from respiratory sites. Evaluation of isolates involved in the multifarm outbreak showed that only two H. parasuis strains were causing disease. These strains ...Continue Reading

References

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Citations

Jan 14, 2003·Veterinary Microbiology·V A de la Puente RedondoE F Rodríguez Ferri
Feb 20, 2003·Veterinary Microbiology·Carol A Lichtensteiger, Eric R Vimr
Feb 8, 2003·Journal of Clinical Microbiology·M L Del RíoE F Rodríguez Ferri
Nov 10, 2010·The Journal of Veterinary Medical Science·Yue-Feng ChuZhong-Xin Lu
Apr 16, 2003·American Journal of Veterinary Research·Simone OliveiraCarlos Pijoan
Jun 7, 2005·Research in Veterinary Science·M L del RíoE F Rodríguez Ferri
May 8, 2010·Veterinary Microbiology·M Cerdà-CuéllarV Aragon
Jan 2, 2009·Comparative Immunology, Microbiology and Infectious Diseases·A J Martín de la FuenteC B Gutiérrez Martín
Aug 27, 2013·Veterinary Microbiology·Bin ZhangHua Yue
Oct 3, 2013·The Veterinary Journal·Mar Costa-Hurtado, Virginia Aragon
Jun 29, 2010·The Veterinary Journal·Nubia Resende MacedoRoberto Maurício Carvalho Guedes
Dec 19, 2006·The Veterinary Journal·Alex OlveraVirginia Aragón
Jun 30, 2005·FEMS Immunology and Medical Microbiology·María Luisa del RíoElías F Rodríguez-Ferri
Mar 2, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Pornchalit AssavacheepConny Turni
Apr 5, 2008·Veterinary Research·Bénédicte BouchetMarcelo Gottschalk
May 20, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christopher S RogersMichael J Welsh
Feb 27, 2021·Veterinary Immunology and Immunopathology·Samantha J HauSusan L Brockmeier
Jan 24, 2017·Veterinary Microbiology·Nubia MacedoMontserrat Torremorell

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