Outer membrane proteins preferentially load MHC class II peptides: implications for a Chlamydia trachomatis T cell vaccine

Vaccine
Karuna P KarunakaranRobert C Brunham

Abstract

CD4 T cell immune responses such as interferon-γ and tumor necrosis factor-α secretion are necessary for Chlamydia immunity. We used an immunoproteomic approach in which Chlamydia trachomatis and Chlamydia muridarum-derived peptides presented by MHC class II molecules on the surface of infected dendritic cells (DCs) were identified by tandem mass spectrometry using bone marrow derived DCs (BMDCs) from mice of different MHC background. We first compared the C. muridarum immunoproteome in C3H mice to that previously identified in C57BL/6 mice. Fourteen MHC class II binding peptides from 11 Chlamydia proteins were identified from C3H infected BMDCs. Two C. muridarum proteins overlapped between C3H and C57B/6 mice and both were polymorphic membrane proteins (Pmps) which presented distinct class II binding peptides. Next we studied DCs from C57BL/6 mice infected with the human strain, C. trachomatis serovar D. Sixty MHC class II binding peptides derived from 27 C. trachomatis proteins were identified. Nine proteins were orthologous T cell antigens between C. trachomatis and C. muridarum and 2 of the nine were Pmps which generated MHC class II binding epitopes at distinct sequences within the proteins. As determined by antigen specif...Continue Reading

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Citations

Jul 15, 2015·Proteomics. Clinical Applications·Allison C Galassie, Andrew J Link
Mar 5, 2016·Expert Review of Vaccines·Hong YuRobert C Brunham
Apr 14, 2016·Journal of Proteomics·Elisabetta GianazzaIvano Eberini
Oct 2, 2015·Mediators of Inflammation·Tamsyn DerrickMartin J Holland
Feb 24, 2017·Clinical and Vaccine Immunology : CVI·Luis M de la MazaRobert C Brunham
Dec 19, 2016·Immunology and Cell Biology·Shradha WaliBernard P Arulanandam
May 29, 2020·Pathogens and Disease·Robert C Brunham, Jorma Paavonen
Nov 26, 2020·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ritu GaurDev Vrat Kamboj
Mar 9, 2021·Expert Review of Vaccines·Luis M de la MazaSukumar Pal
Oct 13, 2020·Sexually Transmitted Diseases·Robert C Brunham
May 26, 2021·Trends in Microbiology·Rupert L Mayer, Francis Impens

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