Outer Membrane Vesicle Proteome of Porphyromonas gingivalis Is Differentially Modulated Relative to the Outer Membrane in Response to Heme Availability

Journal of Proteome Research
Paul D VeithEric C Reynolds

Abstract

Porphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme, which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samples were digested with trypsin and analyzed by tandem mass spectrometry and MaxQuant label-free quantification. In total, 1211 proteins were quantified, with 108 and 49 proteins significantly changing in abundance more than 1.5-fold ( p < 0.05) in the WCLs and OMVs, respectively. The proteins most upregulated in response to heme limitation were those involved in binding and transporting heme, whereas the four proteins most upregulated under the heme-excess condition constitute a putative heme efflux system. In general, the protein abundance ratios obtained for OMVs and WCLs agreed, indicating that changes to the OM protein composition are passed onto OMVs; however, 16 proteins were preferentially packaged into OMVs under one condition mo...Continue Reading

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Citations

Nov 23, 2019·Molecular & Cellular Proteomics : MCP·Daniel Nyberg LarsenPeter Højrup
Sep 19, 2020·Journal of Oral Microbiology·Lin Xin KinEric C Reynolds
Feb 2, 2020·NPJ Biofilms and Microbiomes·Hey-Min Kim, Mary E Davey
Jun 12, 2020·The Journal of Microbiology·Kesavan DineshkumarHuaxi Xu
Dec 19, 2019·Periodontology 2000·Trang NguyenYvonne L Kapila
Jul 17, 2020·Frontiers in Microbiology·Sara Ahmadi BadiAndrea Masotti
Oct 31, 2020·Molecular Oral Microbiology·Paul D VeithEric C Reynolds
Feb 16, 2021·Frontiers in Cellular and Infection Microbiology·Zhiying ZhangYaping Pan
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Daniel Nyberg LarsenPeter Højrup
Mar 12, 2019·Cytokine·Jacqueline E HeathEric C Reynolds
Dec 11, 2021·Journal of Dental Research·P D VeithE C Reynolds
Jan 6, 2022·Microbiology Spectrum·Paul D VeithEric C Reynolds

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