Outer Membrane Vesicles from Neisseria Meningitidis (Proteossome) Used for Nanostructured Zika Virus Vaccine Production

Scientific Reports
Paula MartinsMarcelo Lancellotti

Abstract

The increase of Zika virus (ZIKV) infections in Brazil in the last two years leaves a prophylactic measures on alert for this new and emerging pathogen. Concerning of our positive experience, we developed a new prototype using Neisseria meningitidis outer membrane vesicles (OMV) on ZIKV cell growth in a fusion of OMV in the envelope of virus particles. The fusion of nanoparticles resulting from outer membrane vesicles of N. meningitidis with infected C6/36 cells line were analyzed by Nano tracking analysis (NTA), zeta potential, differential light scattering (DLS), scan and scanning transmission eletronic microscopy (SEM and STEM) and high resolution mass spectometry (HRMS) for nanostructure characterization. Also, the vaccination effects were viewed by immune response in mice protocols immunization (ELISA and inflammatory chemokines) confirmed by Zika virus soroneutralization test. The results of immunizations in mice showed that antibody production had a titer greater than 1:160 as compared to unvaccinated mice. The immune response of the adjuvant and non-adjuvant formulation activated the cellular immune response TH1 and TH2. In addition, the serum neutralization was able to prevent infection of virus particles in the glial ...Continue Reading

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Citations

Nov 25, 2018·Vaccines·Khairunnisa Abdul GhaffarLaurent Renia
Oct 28, 2019·Scientific Reports·Jeany DelafioriRodrigo Ramos Catharino
Jan 21, 2021·Pathogens·Gabriel Augusto Pires de SouzaLuiz Felipe Leomil Coelho
Feb 2, 2021·Acta Tropica·Jonatas Lobato DuarteMarlus Chorilli
Jul 3, 2021·Vaccines·Giuseppe CappellanoAnnalisa Chiocchetti
Aug 10, 2021·International Journal of Biological Macromolecules·Mirza Muhammad Faran Ashraf BaigChengfei Zhang

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Datasets Mentioned

BETA
KU729217

Methods Mentioned

BETA
ELISA
PCR
transmission electron microscopy

Software Mentioned

PLS
DA
MetaboAnalyst

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