PMID: 8944662Nov 1, 1996Paper

Outward K+ current in epithelial cells isolated from intermediate portion of endolymphatic sac of guinea pigs

The American Journal of Physiology
D Wu, N Mori

Abstract

Ion currents in epithelial cells isolated from the intermediate portion of endolymphatic sac (ES) in guinea pigs were investigated with the use of the whole cell patch-clamp technique. Depolarizing voltage steps from a holding potential of -60 mV induced a time- and voltage-dependent outward current, which is comparable to that of delayed rectifying K+ currents. The average resting membrane potential in the current-clamp mode was -54.8 +/- 11 mV (n = 45), which was similar to the value of zero current potential (-55.6 +/- 0.8 mV, n = 32) obtained from current-voltage (I-V) relationships of outward currents in voltage-clamp mode. The I-V relationship of the tail current exhibited a reversal potential (Erev) of -78.1 +/- 0.9 mV (n = 19) in standard external solution. The Erev of the outward current was linearly related to the logarithm of extracellular K+ concentrations. The slope was 48 mV per 10-fold change in extracellular K+ concentrations. The time constants of K+ current activation, inactivation, and K+ tail current deactivation were voltage dependent. The steady-state activation and inactivation of K+ current exhibited a sigmoidal relationship to voltage. The 50% maximal activation voltage and slope factor were -21 and 11 ...Continue Reading

References

Dec 1, 1992·Experimental Eye Research·K CooperJ Rae
Jan 1, 1991·Acta Anatomica·H FurutaS Sakai
Sep 1, 1990·The Journal of Physiology·B A Hughes, R H Steinberg
Jan 1, 1988·The Journal of Physiology·T E DeCourseyM R Silver
May 1, 1989·The Journal of Laryngology and Otology·M Takumida, D Bagger-Sjöbäck
Mar 1, 1988·Acta Oto-laryngologica·F MizukoshiJ Wersäll
Jan 1, 1988·Archives of Oto-rhino-laryngology·P A WackymH Rask-Andersen
Dec 1, 1987·Pflügers Archiv : European journal of physiology·H MatsuuraY Imoto
Jan 1, 1987·Archives of Oto-rhino-laryngology·N MoriC Morgenstern
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Apr 1, 1983·Archives of Oto-rhino-laryngology·H AmanoC Morgenstern
Jul 1, 1993·Circulation Research·C H GelbandJ R Hume
Jun 1, 1994·Pflügers Archiv : European journal of physiology·T Kotera, P D Brown
Jan 1, 1994·Journal of Neurophysiology·K J Rennie, M J Correia
Mar 31, 1993·Biochemical and Biophysical Research Communications·O StraussM Wienrich

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Citations

Oct 29, 1998·The Journal of Biological Chemistry·B Q ShenT F Zioncheck
Aug 5, 2000·Current Opinion in Urology·C F Donatucci
Apr 7, 2004·Médecine sciences : M/S·Vincent CouloignerEvelyne Ferrary
Mar 8, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Indra Ramasamy
Jun 10, 2016·Scientific Reports·Andreea MilasanCatherine Martel
Oct 26, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·V CouloignerE Ferrary
Oct 6, 2000·The Journal of Clinical Investigation·Z G ZhangM Chopp
Oct 7, 2006·Current Opinion in Gastroenterology·D H Alpers
May 1, 2008·Animal : an International Journal of Animal Bioscience·T-J LiM Xie
Nov 3, 2016·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Nozomu MoriHiroshi Hoshikawa
Dec 20, 2003·American Journal of Physiology. Cell Physiology·Laura I EscobarJuan J Bolívar
Dec 6, 2006·Pflügers Archiv : European journal of physiology·Takenori MiyashitaMasahiro Sokabe
Feb 15, 2008·Biochemical and Biophysical Research Communications·Kosuke AkiyamaNozomu Mori

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