Outward potassium currents in freshly isolated smooth muscle cell of dog coronary arteries.

Circulation Research
D W Wilde, K S Lee

Abstract

Outward membrane currents were characterized in single coronary smooth muscle cells of adult beagle dogs. The cells averaged 96.4 x 7.1 microns and had a resting potential of -50.7 mV, an input resistance of 307.9 M omega, a capacitance of 32.3 pF, and a calculated membrane surface area of 4,037 microns2. The cells contracted in response to external application of acetylcholine or high K+. In voltage clamp by use of the suction pipette method, outward current began to appear at -50 mV and reached 15.2 nA at 50 mV with a current density of 376.5 microA/cm2. The current was reduced by external tetraethylammonium, Ba2+, and internal Cs+, and its reversal potential had a Nernst relation to external K+ concentration. Elevation of external Ca2+ (Ca2+o) from 0 to 0.3 mM increased total K+ current by up to 300%; elevation of internal Ca2+ (Ca2+i) to 5 x 10(-7) M by internal perfusion increased total outward current to a similar extent, suggesting a large difference in Ca2+ transmembrane sensitivity. Total whole-cell K+ current consisted of two components: an initial time-independent current (Ii) followed by a time-dependent current (It). Ii and It were through separate K+ channels based on differences in a) sensitivity to Ca2+09b) modu...Continue Reading

References

Jul 1, 1979·The American Journal of Physiology·D R Harder, N Sperelakis
Dec 1, 1985·Pflügers Archiv : European journal of physiology·U Klöckner, G Isenberg
Mar 1, 1986·The Journal of Physiology·J D McCann, M J Welsh
Feb 1, 1987·Pflügers Archiv : European journal of physiology·J V Walsh, J J Singer
Feb 1, 1987·Pflügers Archiv : European journal of physiology·J J Singer, J V Walsh
Apr 1, 1987·The American Journal of Physiology·Y OhyaH Kuriyama
Apr 1, 1987·Pflügers Archiv : European journal of physiology·L Toro, E Stefani
Jan 1, 1988·The Japanese Journal of Physiology·T Tomita
Jun 1, 1989·The Journal of Physiology·J R Hume, N Leblanc
Jun 12, 1987·Science·D M WarshawS S Work
Sep 1, 1984·Pflügers Archiv : European journal of physiology·W BergerW Schwarz
Oct 1, 1982·The Journal of Physiology·J N BarrettB S Pallotta
Jan 1, 1983·The Journal of Membrane Biology·R Latorre, C Miller
Nov 11, 1980·The American Journal of Physiology·J J Singer, J V Walsh
Nov 1, 1980·The Journal of Physiology·A L Gorman, M V Thomas
Feb 1, 1980·Journal of Neuroscience Methods·K S LeeA M Brown

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Citations

Apr 1, 1991·Pflügers Archiv : European journal of physiology·P BonnetD R Harder
May 1, 1994·Pflügers Archiv : European journal of physiology·X Xu, K S Lee
Mar 1, 1990·Pflügers Archiv : European journal of physiology·D R BielefeldJ Krier
Apr 1, 1994·European Journal of Pharmacology·K Obara, H Yabu
Sep 15, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Léna BorbouseJohnathan D Tune
Oct 16, 2010·Cardiovascular Therapeutics·Lakshman Goonetilleke, John Quayle
Apr 21, 2010·Experimental Biology and Medicine·Gregory M Dick, Johnathan D Tune
May 1, 1996·Journal of Cardiovascular Pharmacology·S T O'Rourke
May 30, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Mathieu GautierPierre Bonnet
Jun 1, 1994·The American Journal of Physiology·N LeblancP M Leung

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