Ovalbumin challenge following immunization elicits recruitment of eosinophils but not bronchial hyperresponsiveness in guinea-pigs: time course and relationship to eosinophil activation status

Pulmonary Pharmacology
K H BannerC P Page

Abstract

Eosinophils are known to be present in the airways of allergic asthmatics, and have been suggested to contribute to the pathophysiological changes accompanying this condition, particularly hyperresponsiveness to airway spasmogens. However, a causal relationship between pulmonary eosinophil accumulation and bronchial hyperresponsiveness in asthma is not proven. In the present study, the time course of pulmonary cell influx was investigated in an immunized guinea-pig model. Eosinophil activation status was also determined together with the bronchial responsiveness to histamine. Guinea-pigs were sensitized [20 micrograms ovalbumin (OVA) per animal in A1(OH)3 (0.5 ml) i.p.] and subsequently challenged with aerosolized OVA (100 micrograms/ml) for 1 h 18-21 days later. At different time points (1 h to 72 h) after OVA challenge, bronchial responses to i.v. histamine were measured and bronchoalveolar lavage (BAL) was performed to assess pulmonary cell influx. Eosinophil peroxidase (EPO) and total protein levels were measured in BAL fluid supernatants. Exposure of sensitized animals to aerosolized OVA produced a significant increase (P < 0.05 vs. sham immunized) in eosinophil infiltration 24 h later which was sustained up to 72 h. Despi...Continue Reading

Citations

Mar 29, 2014·The Journal of Pharmacy and Pharmacology·Maelíosa T C McCruddenRyan F Donnelly
Jan 25, 2003·Current Allergy and Asthma Reports·Heidi Isenberg-FeigAndrea Keane-Myers
Jun 14, 2000·European Journal of Pharmacology·R PonsE J Morcillo

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