Ovalbumin Epitope SIINFEKL Self-Assembles into a Supramolecular Hydrogel

Scientific Reports
Meder KamalovChristian F W Becker

Abstract

Here we show that the well-known ovalbumin epitope SIINFEKL that is routinely used to stimulate ovalbumin-specific T cells and to test new vaccine adjuvants can form a stable hydrogel. We investigate properties of this hydrogel by a range of spectroscopic and imaging techniques demonstrating that the hydrogel is stabilized by self-assembly of the peptide into nanofibres via stacking of β-sheets. As peptide hydrogels are known to stimulate an immune response as adjuvants, the immunoactive properties of the SIINFEKL peptide may also originate from its propensity to self-assemble into a hydrogel. This finding requires a re-evaluation of this epitope in adjuvant testing.

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Citations

Nov 5, 2020·Soft Matter·Vinay Kumar Belwal, Nitin Chaudhary

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Methods Mentioned

BETA
X-ray
circular dichroism
nuclear magnetic resonance
rheology
electron
NMR
scanning electron microscopy
transmission electron microscopy

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