Ovarian cancer proliferation and apoptosis are regulated by human transfer RNA methyltransferase 9-likevia LIN9

Oncology Letters
Huai Mei ChenYan Hong Gao

Abstract

Current traditional treatment options have little impact on the long-term survival of patients with ovarian cancer due to a lack of understanding of the molecular transformations that occur in ovarian carcinoma. Transfer RNAs (tRNAs) perform a key role in protein translational fidelity. Enzymes involved in tRNA modification may function as regulators of cancer progression. Human tRNA methyltransferase 9-like (hTRM9L) catalyzes tRNA wobble base modifications, which regulate ovarian cancer growth and apoptosis via the retinoblastoma protein (pRB) and p53 signaling pathways. The aim of the present study was to confirm the role of hTRM9L in the proliferation and apoptosis of ovarian cancer. Immunohistochemistry was performed to investigate the expression of hTRM9L and LIN9 in 70 ovarian tissues. hTRM9L was amplified by polymerase chain reaction (PCR) and inserted into the Ubi-multiple cloning site-enhanced green fluorescent protein (EGFP)-internal ribosome entry site-puromycin lentiviral expression vector to create the Ubi-KIAA1456-EGFP-puromycin (LV-KIAA1456) vector. The lentiviruses were subsequently compounded and transduced into HO8910PM cells. hTRM9L, LIN9 and B-cell lymphoma 2 (Bcl-2)/Bcl-2 associated X protein (Bax) expressi...Continue Reading

References

Dec 4, 2003·Molecular and Cellular Biology·Hamid R Kalhor, Steven Clarke
May 13, 2004·Genes, Chromosomes & Cancer·James M FlanaganGeorgia Chenevix-Trench
Aug 17, 2006·Cancer Research·Zongxiang ZhouAlexander Yu Nikitin
Dec 18, 2007·Molecular Cell·Ulrike BegleyThomas J Begley
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Jan 22, 2014·Anesthesia and Analgesia·Kathryn M Stowell
Jun 9, 2015·Gynecologic Oncology·Helen E DinkelspielJason D Wright
Aug 19, 2015·The Journal of Physical Chemistry Letters·Kong Ooi TanMatthias Ernst
Sep 8, 2015·Cancer Causes & Control : CCC·Gibson Ming Wei GayWei-Yen Lim
Oct 21, 2015·Genomics Data·Fumiaki ObataMasayuki Miura
Nov 12, 2015·British Journal of Cancer·Jelena BeševićMelissa A Merritt

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Citations

Feb 13, 2019·Future Medicinal Chemistry·Lauren EndresRebecca Rose
Jul 1, 2018·Genes·Francesco MorenaSabata Martino
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ge-Lin LiuShi-Shun Liu

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Methods Mentioned

BETA
surgical resection
transfection
PCR
protein assay
flow cytometry

Software Mentioned

Macquit
CellQuest
SPSS

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