Ovarian stimulation reduces IL-6 release from mouse and human pre-implantation embryos

American Journal of Reproductive Immunology : AJRI
Chunhua YuYuanqing Yao

Abstract

In our previous study, ovarian stimulation retarded post-implantation development and caused 92 genes, including interleukin-6 (IL-6), to be differentially expressed in mouse blastocysts. None of them had been tested at protein level, In this study, we confirm the level change of IL-6 mRNA in mouse blastocysts and IL-6 protein release to the culture medium from mouse and human pre-implantation embryos. IL-6 mRNA were measured by PCR and IL-6 release to the culture medium were measured by ELISA. Superovulation caused a 1.95-fold reduction in IL-6 mRNA in mouse blastocysts and reduced IL-6 secretion into the culture medium in mouse zygotes which were developed to the blastocyst stage (6.397 ± 0.635 versus 4.799 ± 0.312 pg/mL). Ovarian stimulation also significantly reduced IL-6 release into the culture medium in human zygotes which were cultured for 2 days (4.713 ± 0.945 versus 4.543 ± 0.796 pg/mL). Our findings indicate that IL-6 might serve as a candidate marker for evaluating embryo developmental competence.

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Citations

Dec 22, 2015·Cell and Tissue Research·Kazuki YamagamiNobuhiko Yamauchi
Nov 28, 2012·American Journal of Reproductive Immunology : AJRI·Michelle Van SinderenEvdokia Dimitriadis
Oct 14, 2017·Clinical and Experimental Reproductive Medicine·Rebecca L Kelley, David K Gardner
Nov 25, 2017·The Journal of Obstetrics and Gynaecology Research·Chunhua YuYuanqing Yao
Sep 25, 2021·The Journal of Obstetrics and Gynaecology Research·Huan Jiang, Jian-Xiong Li

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