Ovate family protein1 interaction with BLH3 regulates transition timing from vegetative to reproductive phase in Arabidopsis

Biochemical and Biophysical Research Communications
Liguo ZhangYing Chang

Abstract

Three-Amino-acid-Loop-Extension(TALE) homeodomain transcription factor BLH3 regulates timing of transition from vegetative to reproductive phase. Previous preliminary results obtained using large-scale yeast two-hybrids indicate that BLH3 protein possibly interact with Ovate Family Proteins(OFPs) transcription co-regulators. Nevertheless, it is uncertain whether OFP1-BLH3 complex is involved in regulation of timing of transition from vegetative to reproductive phase in Arabidopsis. The interaction between BLH3 and OFP1 was re-tested and verified by a yeast two-hybrid system. We found that the BLH3-OFP1 interaction was mainly mediated through the BLH3 homeodomain. Meanwhile, this interaction was further confirmed by bimolecular fluorescence complementation (BiFC) in vivo. Further, by establishing protoplast transient expression, we discovered that BLH3 acts as a transcriptional activator, whereas OFP1 functioned as a repressor. The interactions between OFP1 and BLH3 can reduce BLH3 transcriptional activity. The ofp1 mutant lines and blh3 mutant lines, OFP1 overexpress lines and BLH3 overexpress lines can both influence timing of transition from vegetative to reproductive phase. Furthermore, 35s:OFP1/blh3 plants exhibited floweri...Continue Reading

References

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Citations

Apr 12, 2016·Frontiers in Plant Science·Shucai WangBrian Ellis
Dec 30, 2017·Genome Biology·Yu-Pan ZouYa-Long Guo
Nov 23, 2019·Horticulture Research·Shengen ZhouGuoping Chen
Dec 15, 2019·Current Opinion in Plant Biology·Ashley SnoufferEsther van der Knaap
Feb 12, 2021·Plant Science : an International Journal of Experimental Plant Biology·Haoyuan ChenXinglin Du

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