Ovca1, a candidate gene of the genetic modifier of Tp53, Mop2, affects mouse embryonic lethality

Genes, Chromosomes & Cancer
Min LiangSusan C Evans

Abstract

In this study, we show genetic modifier genes of Tp53 that can exacerbate embryonic abnormalities. Using a mouse model in which CE/J mice were crossed with the Tp53-null 129/Sv (129-Trp53(tm1 Tyj)) mice, a subset of Tp53+/- and -/- male and female embryos died during gestation. Our hypothesis, based on the genotypes of survivors, is that two genetic modifiers and a Tp53 null allele lead to an increase in embryonic lethality. We previously identified a recessive modifier (Mop1) from CE/J mice on chromosome 11 centromeric to Tp53. We have uncovered a dominant modifier (Mop2) from 129/Sv mice telomeric to Tp53. We discovered a polymorphic change (321P-->321S) of Ovca1 within the Mop2 locus of CE/J mice. This polymorphism increased both mRNA and protein levels of OVCA1 in various tissues. CE/J primary cells cultured from different tissues proliferated more rapidly than 129/Sv cells. In addition, CE/J cells cycled while 129/Sv cells had a higher arrest in the G1 phase. Transfection of Ovca1 containing the 321P polymorphism into CE/J cells caused a higher G1 arrest. The pattern of OVCA1 expression also changed from being diffuse throughout the cytoplasm in 129/Sv cells to being punctuate in the cytoplasm of CE/J cells. Tp53+/- abnorm...Continue Reading

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Citations

Jul 13, 2011·Zygote : the Biology of Gametes and Early Embryos·Tomasz LechJozefa Styrna
Jul 28, 2010·Toxicon : Official Journal of the International Society on Toxinology·Ashima K Sharma, David FitzGerald
Oct 1, 2019·American Journal of Physiology. Renal Physiology·Davide P CinàSusan E Quaggin
Mar 5, 2016·Cold Spring Harbor Protocols·Karlyne M Reilly

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