Over-expression of a high-affinity phosphate transporter in transgenic barley plants does not enhance phosphate uptake rates

Functional Plant Biology : FPB
Anne L RaeF W Smith

Abstract

Transgenic barley plants that over-express the gene encoding a phosphate transporter were generated and used to test the hypothesis that manipulation of transporters may lead to improved phosphate uptake by plant roots. Replicate T2 seedlings from a homozygous line with a single locus insertion were grown in dilute flow culture. The phosphate contents and uptake rates of these plants were compared with control transgenic and wild-type plants. When external phosphate concentration was maintained at 10 μM, all plants including the transgenic over-expressing line displayed low rates of phosphate uptake and contained high levels of phosphate in the shoot tissue. When external phosphate concentration was maintained at 2 μM, the uptake rates increased to a similar level in all plant lines. Three transgenic over-expressing lines were then grown in soil at a range of phosphate concentrations and the dry weights and total phosphorus contents of the shoots were measured and compared to a transgenic control line. The results showed that over-expression of the gene encoding a phosphate transporter did not improve the uptake of phosphate under any of the conditions tested. Transporter activity is likely to be influenced by post-transcriptio...Continue Reading

Citations

Nov 2, 2012·AoB Plants·K K Vinod, Sigrid Heuer
Mar 12, 2011·Annual Review of Plant Biology·Sally E Smith, F Andrew Smith
May 15, 2013·Molecular Biology Reports·Hai-Jian LinGuang-Tang Pan
Mar 29, 2012·Plant, Cell & Environment·Jakob SantnerFien Degryse
Aug 18, 2012·Cellular and Molecular Life Sciences : CMLS·Ajay JainKashchandra G Raghothama
Jun 3, 2017·Frontiers in Plant Science·Duoliya WangYinxin Li
Aug 29, 2013·BioMed Research International·Keisuke MatsuiYoshikazu Tanaka
Aug 19, 2007·Plant Molecular Biology·Abdelbagi M IsmailMatthias Wissuwa
Aug 18, 2006·Colloids and Surfaces. B, Biointerfaces·Hong-Bo ShaoJiang-Feng Cheng

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