Over-expression of CXCR4 on mesenchymal stem cells augments myoangiogenesis in the infarcted myocardium.

Journal of Molecular and Cellular Cardiology
Dongsheng ZhangYigang Wang

Abstract

Bone marrow mesenchymal stem cells (MSCs) participate in myocardial repair following myocardial infarction. However, their in vivo reparative capability is limited due to lack of their survival in the infarcted myocardium. To overcome this limitation, we genetically engineered male rat MSCs overexpressing CXCR4 in order to maximize the effect of stromal cell-derived factor-1alpha (SDF-1alpha) for cell migration and regeneration. MSCs were isolated from adult male rats and cultured. Adenoviral transduction was carried out to over-express either CXCR4/green fluorescent protein (Ad-CXCR4/GFP) or Ad-null/GFP alone (control). Flow cytometry was used to identify and isolate GFP/CXCR4 over-expressing MSCs for transplantation. Female rats were assigned to one of four groups (n=8 each) to receive GFP-transduced male MSCs (2 x 10(6)) via tail vein injection 3 days after ligation of the left anterior descending (LAD) coronary artery: GFP-transduced MSCs (Ad-null/GFP-MSCs, group 1) or MSCs over-expressing CXCR4/GFP (Ad-CXCR4/GFP-MSCs, group 2), or Ad-CXCR4/GFP-MSCs plus SDF-1alpha (50 ng/microl) (Ad-CXCR4/GFP-MSCs/SDF-1alpha, group 3), or Ad-miRNA targeting CXCR4 plus SDF-1alpha (Ad-miRNA/GFP-MSCs+SDF-1alpha treatment, group 4). Cardiodyna...Continue Reading

References

Sep 10, 1998·The Journal of Cell Biology·Z QuJ Huard
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·K KawabataT Nagasawa
Jul 17, 2003·The Journal of Clinical Investigation·Orit KolletTsvee Lapidot
Jul 10, 2004·Circulation Research·Mark F Pittenger, Bradley J Martin
Nov 4, 2004·Journal of Molecular and Cellular Cardiology·Yigang WangMuhammad Ashraf
Feb 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bilal B AyachPeter P Liu
Feb 24, 2006·European Journal of Haematology·Nancy Van Overstraeten-SchlögelAndré Gothot
Aug 24, 2006·Journal of Molecular and Cellular Cardiology·Yigang WangMuhammad Ashraf
Oct 4, 2006·The Journal of Clinical Investigation·Michelle B BowieConnie J Eaves
Dec 26, 2006·Journal of Molecular and Cellular Cardiology·Meifeng XuMuhammad Ashraf
Jan 9, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Yigang WangMuhammad Ashraf
May 15, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ming ZhangMarc S Penn

❮ Previous
Next ❯

Citations

Nov 7, 2013·Stem Cells International·Abhishek Sohni, Catherine M Verfaillie
Jun 3, 2014·BMC Cell Biology·Rebecca A PelekanosNicholas M Fisk
Mar 16, 2011·Cardiology Research and Practice·S Van LinthoutC Tschöpe
Jun 7, 2008·Circulation Research·Stefanie Dimmeler, Annarosa Leri
Feb 4, 2009·Antioxidants & Redox Signaling·Jeremy L HerrmannDaniel R Meldrum
May 23, 2009·Circulation Research·Marc S Penn
Jul 3, 2009·Journal of Vascular Research·Chandan K SenSashwati Roy
Nov 10, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Yigang WangAmbikaipakan Balasubramaniam
Feb 20, 2010·Circulation Research·John V TerrovitisEduardo Marbán
Jul 9, 2010·World Journal of Gastroenterology : WJG·Xue-Jun DongJian-Zhong Shao
Aug 31, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Dongsheng ZhangYigang Wang
Sep 8, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Marcello Rota
May 28, 2011·Circulation Research·Konstantinos E HatzistergosJoshua M Hare
Sep 16, 2011·Regenerative Medicine·Nicholas A KourisBrenda M Ogle
Jan 7, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Xiao-Feng LeGeorge A Calin
Apr 20, 2011·Circulation Journal : Official Journal of the Japanese Circulation Society·Hui XuQian Zhang
Feb 2, 2012·Circulation Journal : Official Journal of the Japanese Circulation Society·Masayuki KuboKimikazu Hamano
Jan 12, 2011·Expert Opinion on Biological Therapy·Ting Ting Lau, Dong-An Wang
Nov 10, 2010·Expert Opinion on Biological Therapy·Timothy J MyersAnna Spagnoli
Feb 10, 2011·Expert Opinion on Biological Therapy·Darryl R Davis, Duncan J Stewart
Aug 9, 2011·Experimental Diabetes Research·Stella BernardiPaola Secchiero
Sep 30, 2015·Journal of Cellular and Molecular Medicine·Fatemeh PourrajabMohamad Reza Zare-Khormizi
Jan 10, 2012·The Journal of Surgical Research·Todd J WannemuehlerDaniel R Meldrum
Sep 29, 2011·Veterinary Immunology and Immunopathology·Naoki ChimuraSadatoshi Maeda
Mar 5, 2011·Advanced Drug Delivery Reviews·Zhaoyang YeWensong Tan
Mar 4, 2011·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·S RouxH Rouard
Feb 4, 2011·Experimental Hematology·Ling GuoYaojiong Wu
Nov 26, 2009·Cytotherapy·Richard O Cannon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Cardiac Remodeling

Cardiac remodeling in response to a myocardial infarction is characterized by progressive ventricular dilatation, cardiac hypertrophy, fibrosis, and deterioration of cardiac performance. Discover the latest research on Cardiac Remodeling here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.