Over-expression of FSIP1 promotes breast cancer progression and confers resistance to docetaxel via MRP1 stabilization

Cell Death & Disease
Meisi YanZhigao Li

Abstract

Fibrous sheath-interacting protein 1 (FSIP1) functions centrally in breast carcinogenesis and progression, although its exact role remains to be clarified. Therefore, we sought to establish a correlation between the clinico-pathological features of breast cancer and FSIP1 expression in breast cancer tissues, as well as to validate its role in tumor progression and chemo-resistance. We analyzed FSIP1 expression in the breast cancer and para-tumor tissues by immunohistochemistry. We performed MTT, Caspase-Glo 3/7 Assay, Annexin V staining, wound healing and trans-well assays to evaluate cellular apoptosis, proliferation, migration and invasion in FSIP1 knockout and wild-type breast cancer cell lines. Additionally, we examined the effects of FSIP1 on docetaxel sensitivity in a nude mice model transplanted with control or FSIP1 knockout breast cancer cells, and also evaluate its role in tumor metastasis. FSIP1 and MRP1 interaction was determined by co-immunoprecipitation and mass spectrometry. We found that breast cancer cells and tissues consistently demonstrated elevated FSIP1 expressions, which correlated with poor overall survival. Notably, patients with high FSIP1 expression in their tumors undergoing docetaxel neoadjuvant che...Continue Reading

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Citations

Nov 13, 2019·Molecular Carcinogenesis·Xuelu LiMan Li
Jul 24, 2020·World Journal of Gastrointestinal Oncology·Hui-Ying WuDong-Hua Geng
Nov 17, 2020·Frontiers in Oncology·Dan ZhangNi Xie

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Methods Mentioned

BETA
xenograft
electrophoresis
X-ray
flow cytometry
Co-IP
bioluminescence imaging

Software Mentioned

Graphpad Prism
JME Pro10

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