Over-expression of ΔNp63α facilitates rat corneal wound healing in vivo

Bioscience, Biotechnology, and Biochemistry
Jeng-Yuan Yao, Jan-Kan Chen

Abstract

To investigate the roles of ΔNp63α during corneal wound healing and the genes regulated by ΔNp63α in limbal epithelial cells. Adenovirus or shRNA targeting ΔNp63α were pre-injected into the anterior chamber of rat eyeballs and the central corneal epithelium was then wounded with NaOH. The effects of ΔNp63α expression during wound healing were observed by propidium iodide staining. In addition, limbal epithelial cells were cultured and ectopically expressed ΔNp63α by transfecting Ad-ΔNp63α. Total RNA was extracted from transfected epithelial cells and subjected to a gene expression microarray assay. The results showed that over-expression of ΔNp63α accelerated the process of corneal wound healing while knockdown of ΔNp63α impaired the process. ΔNp63α positively up-regulated several cell growth promoter genes and could be referred as a positive regulator of limbal epithelial cell proliferation. It might also inhibit cell differentiation and cell death by differential target gene regulation.

References

Sep 1, 1992·Archives of Ophthalmology·T NishidaM Hikida
Feb 1, 1994·The Journal of Cell Biology·S M Frisch, H Francis
Apr 1, 1996·Cell Stress & Chaperones·M TavariaR L Anderson
Oct 23, 1997·Current Opinion in Cell Biology·S M Frisch, E Ruoslahti
May 20, 1998·Current Biology : CB·S A MarstersA Ashkenazi
May 23, 1998·Annual Review of Immunology·D T Chao, S J Korsmeyer
Dec 5, 1998·American Journal of Human Genetics·H Semb, G Christofori
Jul 13, 2000·The New England Journal of Medicine·R J TsaiJ K Chen
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PellegriniM De Luca
Oct 20, 2001·The Journal of Biological Chemistry·Yasushi SasakiTakashi Tokino
Apr 25, 2002·Nature Reviews. Genetics·Elaine Fuchs, Srikala Raghavan
Jun 18, 2003·The Journal of Cell Biology·Alice S T Wong, Barry M Gumbiner
Aug 19, 2003·Genes & Development·Charles K KaufmanElaine Fuchs
Mar 31, 2005·Cell·Rebecca A IhrieLaura D Attardi
Jun 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Enzo Di IorioMichele De Luca
Mar 7, 2006·Biochemical and Biophysical Research Communications·Wing-Keung ChuJan-Kan Chen
Nov 10, 2006·Journal of Cell Science·Eleonora CandiGerry Melino
May 1, 2007·Regenerative Medicine·Julie T DanielsShankar Seetharaman
May 22, 2007·Annals of the New York Academy of Sciences·Kevin A Morano
Jun 15, 2007·The Journal of Cell Biology·Vanessa BarbaroMichele De Luca
Jul 13, 2007·Stem Cell Reviews·Julie T DanielsChris Mason
Jan 17, 2008·The Journal of Biological Chemistry·Wing-Keung ChuJan-Kan Chen
Nov 13, 2008·Cytotechnology·Finbarr O'Sullivan, Martin Clynes
Jun 25, 2010·The New England Journal of Medicine·Paolo RamaGraziella Pellegrini
Jan 30, 2014·Veterinary Ophthalmology·Maresuke MoritaRyohei Nishimura
Feb 19, 2017·Biochemical and Biophysical Research Communications·Eleonora CandiGerry Melino

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
protein assay
transfection
flow cytometry
Infection
protein folding

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
F E Kruse, S C Tseng
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
H HongP Zhu
Investigative Ophthalmology & Visual Science
Wakako AdachiJoram Piatigorsky
Zhongguo shi yan xue ye xue za zhi
Xiao-Ming GuoBing-An Feng
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology
Z PanB Sun
© 2022 Meta ULC. All rights reserved