Over-expression of the rice OsAMT1-1 gene increases ammonium uptake and content, but impairs growth and development of plants under high ammonium nutrition

Functional Plant Biology : FPB
Mohammad S HoqueNarayana M Upadhyaya

Abstract

A transgenic approach was undertaken to investigate the role of a rice ammonium transporter (OsAMT1-1) in ammonium uptake and consequent ammonium assimilation under different nitrogen regimes. Transgenic lines overexpressing OsAMT1-1 were produced by Agrobacterium-mediated transformation of two rice cultivars, Taipei 309 and Jarrah, with an OsAMT1-1 cDNA gene construct driven by the maize ubiquitin promoter. Transcript levels of OsAMT1-1 in both Taipei 309 and Jarrah transgenic lines correlated positively with transgene copy number. Shoot and root biomass of some transgenic lines decreased during seedling and early vegetative stage compared to the wild type, especially when grown under high (2 mm) ammonium nutrition. Transgenic plants, particularly those of cv. Jarrah recovered in the mid-vegetative stage under high ammonium nutrition. Roots of the transgenic plants showed increased ammonium uptake and ammonium content. We conclude that the decreased biomass of the transgenic lines at early stages of growth might be caused by the accumulation of ammonium in the roots owing to the inability of ammonium assimilation to match the greater ammonium uptake.

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Citations

Sep 3, 2020·Plant Biotechnology Journal·Jie WuCheng-Bin Xiang
Apr 19, 2017·Journal of Experimental Botany·Hua LiChengcai Chu
Sep 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Weibo XieQifa Zhang
Mar 5, 2016·Journal of Experimental Botany·Akimasa SasakiJian Feng Ma
Dec 20, 2018·Plant Biotechnology Journal·Mark Christian Felipe R RedillasJu-Kon Kim
May 1, 2020·Frontiers in Plant Science·Dujun WangXingming Lian
Dec 1, 2014·Plant Cell Reports·Shunying YangYanhua Su
Jun 7, 2018·Nature Reviews. Genetics·Rod A WingQifa Zhang
Jan 11, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhihua ZhangChengcai Chu
May 1, 2006·Functional Plant Biology : FPB·Anshuman KumarAnthony D M Glass
Jan 10, 2012·Annual Review of Plant Biology·Guohua XuAnthony J Miller
Dec 3, 2014·The Plant Journal : for Cell and Molecular Biology·Yangjun ZhangChuanqing Sun
May 6, 2020·The Plant Journal : for Cell and Molecular Biology·Sichul LeeHong Gil Nam
Dec 19, 2006·Plant Physiology·Lixing YuanNicolaus von Wirén

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Datasets Mentioned

BETA
AF289479

Methods Mentioned

BETA
transgenic
in vitro transcription
phosphotransferase
PCR

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