Over-expression of tobacco UBC1 encoding a ubiquitin-conjugating enzyme increases cadmium tolerance by activating the 20S/26S proteasome and by decreasing Cd accumulation and oxidative stress in tobacco (Nicotiana tabacum)

Plant Molecular Biology
Ramin BahmaniSeongbin Hwang

Abstract

Ubiquitin (Ub)-conjugating enzyme (UBC, E2) receives Ub from Ub-activating enzyme (E1) and transfers it to target proteins, thereby playing a key role in Ub/26S proteasome-dependent proteolysis. UBC has been reported to be involved in tolerating abiotic stress in plants, including drought, salt, osmotic and water stresses. To isolate the genes involved in Cd tolerance, we transformed WT (wild-type) yeast Y800 with a tobacco cDNA expression library and isolated a tobacco cDNA, NtUBC1 (Ub-conjugating enzyme), that enhances cadmium tolerance. When NtUBC1 was over-expressed in tobacco, cadmium tolerance was enhanced, but the Cd level was decreased. Interestingly, 20S proteasome activity was increased and ubiquitinated protein levels were diminished in response to cadmium in NtUBC1 tobacco. By contrast, proteasome activity was decreased and ubiquitinated protein levels were slightly enhanced by Cd treatment in control tobacco, which is sensitive to Cd. Moreover, the oxidative stress level was induced to a lesser extent by Cd in NtUBC1 tobacco compared with control plants, which is ascribed to the higher activity of antioxidant enzymes in NtUBC1 tobacco. In addition, NtUBC1 tobacco displayed a reduced accumulation of Cd compared with...Continue Reading

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Citations

Sep 23, 2018·International Journal of Molecular Sciences·Guang ChenLi Zhu
Oct 3, 2018·Environmental Science and Pollution Research International·Raimondas ŠiukštaTatjana Čėsnienė
Apr 25, 2020·International Journal of Molecular Sciences·Weigang LiuHuaijun Si
Mar 17, 2020·Functional Plant Biology : FPB·Liqiang JiaShu Chen
Jul 18, 2017·Redox Biology·Maria LefakiNiki Chondrogianni
Apr 3, 2021·International Journal of Biological Macromolecules·Shambhavi SharmaManoj Prasad
Jan 14, 2020·Ecotoxicology and Environmental Safety·Ramin BahmaniMohammad Reza Bihamta

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Datasets Mentioned

BETA
EB428626
AB505625
AB201240
AB201241

Methods Mentioned

BETA
ubiquitination
transgenic
MDA
PCR
Assay

Software Mentioned

ImageJ
Image J
SAS

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