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Over-expression, purification, and characterization of aminopeptidase N from Escherichia coli

Protein Expression and Purification

Dec 29, 2005

Frank C GolichMichael W Crowder

Abstract

The gene from Escherichia coli encoding aminopeptidase N (PepN) was subcloned into pET-26b, and PepN was over-expressed in BL21(DE3) E. coli and purified using Q-Sepharose chromatography. This protocol yielded over 17 mg of purified, recombinant PepN per liter of growth culture under op...read more

Mentioned in this Paper

Surface Enhanced Laser Desorption Ionization Mass Spectrometry
Fast Protein Liquid Chromatography
Phosphate buffers
Bacterial Proteins
Alkalescens-Dispar Group
Enzymes, antithrombotic
Chromatography
Chelator (genus)
Streptococcus thermophilus
Promoter
Paper Details
References
  • References16
  • Citations6
12
  • References16
  • Citations6
1

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Over-expression, purification, and characterization of aminopeptidase N from Escherichia coli

Protein Expression and Purification

Dec 29, 2005

Frank C GolichMichael W Crowder

PMID: 16380266

DOI: 10.1016/j.pep.2005.11.012

Abstract

The gene from Escherichia coli encoding aminopeptidase N (PepN) was subcloned into pET-26b, and PepN was over-expressed in BL21(DE3) E. coli and purified using Q-Sepharose chromatography. This protocol yielded over 17 mg of purified, recombinant PepN per liter of growth culture under op...read more

Mentioned in this Paper

Surface Enhanced Laser Desorption Ionization Mass Spectrometry
Fast Protein Liquid Chromatography
Phosphate buffers
Bacterial Proteins
Alkalescens-Dispar Group
Enzymes, antithrombotic
Chromatography
Chelator (genus)
Streptococcus thermophilus
Promoter

Similar Papers Found In These Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Molecular Bacteriology

Molecular bacteriology involves fundamental, translational and application-oriented research on the very diverse roles of bacteria in human health and disease. Discover the latest on molecular bacteriology here.

Related Papers

Biochemical and Biophysical Research Communications

Characterization and role of Peptidase N from Salmonella enterica serovar Typhimurium

Biochemical and Biophysical Research CommunicationsJanuary 2, 2007
Anujith Kumar, Dipankar Nandi
Archives of Biochemistry and Biophysics

Structured and catalytic properties of peptidase N from Escherichia coli K-12

Archives of Biochemistry and BiophysicsFebruary 1, 1982
M T McCaman, M R Villarejo
Paper Details
References
  • References16
  • Citations6
12
  • References16
  • Citations6
1
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