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Over-expression, purification, and characterization of aminopeptidase N from Escherichia coli

Protein Expression and Purification

Jun 1, 2006

Frank C GolichMichael W Crowder

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Abstract

The gene from Escherichia coli encoding aminopeptidase N (PepN) was subcloned into pET-26b, and PepN was over-expressed in BL21(DE3) E. coli and purified using Q-Sepharose chromatography. This protocol yielded over 17 mg of purified, recombinant PepN per liter of growth culture under op...read more

Mentioned in this Paper

Cysteine
Sodium Chloride
Gene Expression
Enzymes, antithrombotic
Chelator (genus)
Enzymes, peripheral vasodilators
Centrifugation
Phenanthrolines
Substrate Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Paper Details
References
    • References16
    • Citations6
    • References16
    • Citations6
  • Over-expression, purification, and characterization of aminopeptidase N from Escherichia coli

    Protein Expression and Purification

    Jun 1, 2006

    Frank C GolichMichael W Crowder

    PMID: 16380266

    DOI: 10.1016/j.pep.2005.11.012

    Abstract

    The gene from Escherichia coli encoding aminopeptidase N (PepN) was subcloned into pET-26b, and PepN was over-expressed in BL21(DE3) E. coli and purified using Q-Sepharose chromatography. This protocol yielded over 17 mg of purified, recombinant PepN per liter of growth culture under op...read more

    Mentioned in this Paper

    Cysteine
    Sodium Chloride
    Gene Expression
    Enzymes, antithrombotic
    Chelator (genus)

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    Paper Details
    References
    • References16
    • Citations6
    • References16
    • Citations6
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