Over-production of a glycoside hydrolase family 50 beta-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product

Biotechnology Letters
Dong-Geun LeeSang-Hyeon Lee

Abstract

The gene for beta-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than that in E. coli. The enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose from agar. Neoagarooligosaccharides produced by the enzyme had a whitening effect and inhibited tyrosinase activity in the murine melanoma cell line, B16F10. Neoagarooligosaccharides were not cytotoxic to B16F10 or normal cells. beta-Agarase could therefore be a good whitening, cosmetic additive.

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Citations

Apr 25, 2012·Applied Microbiology and Biotechnology·Won-Jae ChiSoon-Kwang Hong
Sep 20, 2012·Applied Microbiology and Biotechnology·Wei XieZhong Hu
Jun 2, 2009·Marine Biotechnology·Mengxian LongXun Xu
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Feb 10, 2020·Applied Microbiology and Biotechnology·Si Hyoung ParkSoon-Kwang Hong
Oct 5, 2021·Applied Microbiology and Biotechnology·Chaonan DongZhong Hu

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