PMID: 8605299Feb 1, 1996Paper

Over-production of the D1:2 protein makes Synechococcus cells more tolerant to photoinhibition of photosystem II

Plant Molecular Biology
A SoitamoE M Aro

Abstract

Over-expression of the psbAIII gene encoding for the D1 protein (form II; D1:2) of the photosystem II reaction centre in the Synechococcus sp. PCC 7942 was studied using a tac promoter and the lacIQ system. Over-expression was induced with 40 microgram/ml IPTG in the growth medium for either 6 or 12 h at growth irradiance (50 mumol photons m-2 s-1). This treatment doubled the amount of psbAII/III mRNA and the D1:2 protein in membranes but decreased the amount of psbAI messages and the D1:1 protein. The total amount of both heterodimeric reaction centre proteins, D1 and D2, remained constant under growth light conditions, indicating that the number of PSII centres in the membranes was not affected, only the form of the D1 protein was changed from D1:1 to D1:2 in most centres. When the cells were photoinhibited either at 500 or 1000 mumol photons m-2 s-1, in the presence or absence of the protein synthesis inhibitor lincomycin, the D1:2 protein remained at a higher level in cells in which over-expression had been induced by IPTG. These cells were also less prone to photoinhibition of PSII. It is suggested that the tolerance of cells to photoinhibition increases when most PSII reaction centres contain the D1:2 protein at the begin...Continue Reading

References

Jul 1, 1989·Journal of Bacteriology·M R Schaefer, S S Golden
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R J DebusL McIntosh
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A K ClarkeG Oquist
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Jun 4, 1962·Biochemical and Biophysical Research Communications·K SCHERRER, J E DARNELL
May 1, 1977·Plant Physiology·V S RaoJ Myers

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Citations

Aug 1, 2009·Cellular and Molecular Life Sciences : CMLS·Paula MuloEva-Mari Aro
Apr 20, 2006·Photochemistry and Photobiology·Josée Nina BouchardDouglas A Campbell
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·F PartenskyD Vaulot

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