PMID: 11912010Mar 26, 2002Paper

Overactivation of IL-4-induced activator protein-1 in atopic dermatitis

Journal of Dermatological Science
Fumie YamazakiTadashi Tezuka

Abstract

Atopic dermatitis is regarded as mediated by Th2-type immunity. In fact, it frequently coincides with the elevation of immunoglobulin (Ig)-E in patients' sera. Due to the pivotal role of interleukin (IL)-4 in regulation of IgE, we hypothesized if atopic dermatitis represents a hyper-reactive condition in response to IL-4 when it coincides the higher serum level of IgE. To address this possibility, peripheral blood mononuclear cells (PBMC) isolated from patients with atopic dermatitis with the high serum IgE level, from those with psoriasis or from healthy volunteers were stimulated with recombinant IL-4 and analyzed for activation of transcription factors including activator protein (AP)-1 or signal transducers and activators of transcription (STAT)-6 by employing electrophoretic mobility shift assays. Although no significant difference between atopy patients and other groups was observed in the STAT-6 binding activity in IL-4-stimulated PBMC, it over-activated the binding of AP-1 in PBMC of the patients with atopic dermatitis. The AP-1 binding was interfered by the use of an antibody directed against JunB. This is the indication that IL-4-overactivated AP-1 is composed of JunB. Furthermore, semi-quantitative RT-PCR analyses re...Continue Reading

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Citations

Dec 6, 2006·Archives of Dermatological Research·Mohammad Said AshenagarTadashi Tezuka
Nov 29, 2002·The EMBO Journal·Bettina HartensteinPeter Angel
Feb 10, 2007·International Archives of Allergy and Immunology·Tzvetanka Petrova MarkovaHideaki Higashino
Jan 25, 2005·Bioscience, Biotechnology, and Biochemistry·Koichi OgawaNobuyuki Nakamura
Aug 20, 2003·The Journal of Investigative Dermatology·Akira MaedaYoshinori Aragane

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Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.