Overcoming drug-resistant lung cancer by paclitaxel loaded tetrahedral DNA nanostructures

Nanoscale
X XieY Lin

Abstract

Paclitaxel (PTX) is an effective drug against diseases such as lung cancer, ovarian cancer, and breast cancer. However, multidrug resistance limits the clinical applications of this drug. Tetrahedral DNA nanostructures (TDNs) offer great promise as a drug delivery candidate. In our study, we prepared TDNs that were subsequently loaded with PTX (PTX/TDNs). The cytotoxicity of PTX/TDNs and PTX alone on non-small cell lung cancer (NSCLC) cells (A549) and the PTX-resistant cell line (A549/T) was determined using a cell count kit-8 (CCK-8) assay. PTX/TDNs exerted strong lethality on both cell lines. Moreover, drug resistance was overcome. Furthermore, the mechanisms used by PTX/TDNs to overcome drug resistance were studied. The expression of mdr 1 gene and P-glycoprotein (P-gp) in A549/T was found to be downregulated, thus indicating that TDNs serve as a P-gp inhibitor. We also showed that PTX/TDNs killed cancer cells via apoptosis. Thus, PTX/TDNs have great potential for use as a nanodelivery system for the treatment of PTX-resistant NSCLC.

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Citations

Oct 21, 2018·Cell Proliferation·Yanyan XiaNongyue He
Mar 8, 2019·Advanced Healthcare Materials·Divita Mathur, Igor L Medintz
Apr 19, 2019·Cell Proliferation·Yuanyuan WeiBofeng Zhu
Aug 30, 2019·Biotechnology Journal·Yuxi ZhanYunfeng Lin
Jun 27, 2020·Cancer Science·Ratchanee DuangratThaned Kangsamaksin
Dec 12, 2020·Advanced Science·Fay NicolsonSuchetan Pal
Jun 9, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Elise LepeltierCatherine Passirani
Apr 10, 2021·Biomaterials·Yachao ZhangShuangying Gui
Nov 21, 2020·Drug Discovery Today·Bo ChenGang Guo
Jun 5, 2021·Advanced Healthcare Materials·Luyao ShenYonggang Ke
Apr 21, 2018·ACS Applied Materials & Interfaces·Haoran ZhaoLeilei Tian
Jan 31, 2019·ACS Applied Materials & Interfaces·Lingxian MengYunfeng Lin
Mar 30, 2019·ACS Applied Materials & Interfaces·Yuxi ZhanYunfeng Lin
Dec 19, 2018·ACS Applied Materials & Interfaces·Chenchen MaoYunfeng Lin
Sep 26, 2020·ACS Applied Materials & Interfaces·Tao ZhangYunfeng Lin
Jul 3, 2018·ACS Applied Materials & Interfaces·Juanjuan YangBaoquan Ding
Jun 22, 2018·ACS Applied Materials & Interfaces·Xiaoru ShaoYunfeng Lin

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Methods Mentioned

BETA
transfection
electrophoresis
PCR
transmission electron microscopy
dynamic light scattering
Fluorescence microscopy

Software Mentioned

SPSS
BLAST

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