Overcoming factors limiting high-solids fermentation of lignocellulosic biomass to ethanol

Proceedings of the National Academy of Sciences of the United States of America
Thanh Yen NguyenCharles E Wyman

Abstract

Simultaneous saccharification and fermentation (SSF) of solid biomass can reduce the complexity and improve the economics of lignocellulosic ethanol production by consolidating process steps and reducing end-product inhibition of enzymes compared with separate hydrolysis and fermentation (SHF). However, a long-standing limitation of SSF has been too low ethanol yields at the high-solids loading of biomass needed during fermentation to realize sufficiently high ethanol titers favorable for more economical ethanol recovery. Here, we illustrate how competing factors that limit ethanol yields during high-solids fermentations are overcome by integrating newly developed cosolvent-enhanced lignocellulosic fractionation (CELF) pretreatment with SSF. First, fed-batch glucose fermentations by Saccharomyces cerevisiae D5A revealed that this strain, which has been favored for SSF, can produce ethanol at titers of up to 86 g⋅L-1 Then, optimizing SSF of CELF-pretreated corn stover achieved unprecedented ethanol titers of 79.2, 81.3, and 85.6 g⋅L-1 in batch shake flask, corresponding to ethanol yields of 90.5%, 86.1%, and 80.8% at solids loadings of 20.0 wt %, 21.5 wt %, and 23.0 wt %, respectively. Ethanol yields remained at over 90% despite...Continue Reading

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Citations

Feb 15, 2020·Journal of the Science of Food and Agriculture·Ana Belén DíazAna Blandino
Apr 27, 2018·Bioengineering·Augusto C M SouzaKurt A Rosentrater
Oct 19, 2019·Biotechnology for Biofuels·Oscar Rosales-Calderon, Valdeir Arantes
Jan 31, 2020·Biotechnology for Biofuels·Jie WangDominic Sauvageau
Mar 18, 2020·Microbial Cell Factories·Aritha DornauSimon J McQueen-Mason
Mar 27, 2019·Biotechnology for Biofuels·Xiaolin LuoLi Shuai
Jul 9, 2020·Proceedings of the National Academy of Sciences of the United States of America·Sai Venkatesh PingaliLoukas Petridis
Feb 9, 2021·Bioresource Technology·Ramkrishna SinghVijay Singh
Apr 25, 2021·Biotechnology for Biofuels·Peng NingJianming Yang
Jul 16, 2019·Journal of the American Chemical Society·Abhishek S PatriCharles M Cai

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