Abstract
Simultaneous saccharification and fermentation (SSF) of solid biomass can reduce the complexity and improve the economics of lignocellulosic ethanol production by consolidating process steps and reducing end-product inhibition of enzymes compared with separate hydrolysis and fermentation (SHF). However, a long-standing limitation of SSF has been too low ethanol yields at the high-solids loading of biomass needed during fermentation to realize sufficiently high ethanol titers favorable for more economical ethanol recovery. Here, we illustrate how competing factors that limit ethanol yields during high-solids fermentations are overcome by integrating newly developed cosolvent-enhanced lignocellulosic fractionation (CELF) pretreatment with SSF. First, fed-batch glucose fermentations by Saccharomyces cerevisiae D5A revealed that this strain, which has been favored for SSF, can produce ethanol at titers of up to 86 g⋅L-1 Then, optimizing SSF of CELF-pretreated corn stover achieved unprecedented ethanol titers of 79.2, 81.3, and 85.6 g⋅L-1 in batch shake flask, corresponding to ethanol yields of 90.5%, 86.1%, and 80.8% at solids loadings of 20.0 wt %, 21.5 wt %, and 23.0 wt %, respectively. Ethanol yields remained at over 90% despite...Continue Reading
References
Jan 1, 1990·Critical Reviews in Biotechnology·T D'AmoreG G Stewart
Oct 9, 1999·Biotechnology Progress·L R LyndT U Gerngross
Dec 12, 2001·Biotechnology Progress·A R EsteghlalianJ N Saddler
Aug 2, 2003·Biotechnology Progress·Anders WingrenGuido Zacchi
Aug 23, 2005·Bioresource Technology·Todd A Lloyd, Charles E Wyman
Feb 27, 2007·Trends in Biotechnology·Charles E Wyman
Mar 15, 1991·Science·L R LyndC E Wyman
May 13, 2008·Biotechnology for Biofuels·Hiroyuki InoueShigeki Sawayama
Oct 7, 2008·Applied Biochemistry and Biotechnology·Jan B KristensenHenning Jørgensen
Mar 26, 2009·Biotechnology Progress·Yulin LuNathan S Mosier
Dec 17, 2009·Bioresource Technology·W ZhuX J Pan
Oct 23, 2010·Biotechnology and Bioengineering·Noppadon SathitsuksanohY-H Percival Zhang
Sep 15, 2011·Biotechnology and Bioengineering·Q Q WangJ Y Zhu
Nov 19, 2011·Biotechnology and Bioengineering·Daniel Klein-MarcuschamerHarvey W Blanch
Nov 25, 2011·Enzyme and Microbial Technology·Xiaolin Luo, J Y Zhu
Oct 9, 2012·Biotechnology and Bioengineering·Rajeev KumarCharles E Wyman
Sep 12, 2013·Biotechnology and Bioengineering·David Cannella, Henning Jørgensen
Oct 4, 2014·Science·Clint Cheng, Katy C Kao
Oct 4, 2014·Science·Felix H LamGregory Stephanopoulos
Jan 1, 2013·SpringerPlus·Sutticha Na-Ranong ThammasittirongMalee Srisodsuk
Feb 14, 2015·ChemSusChem·Thanh Yen NguyenCharles E Wyman
Citations
Sep 14, 2019·Biotechnology for Biofuels·Aditya BhallaDavid B Hodge
Jan 30, 2020·Biotechnology for Biofuels·Zhiwei GongYi Liu
Nov 11, 2019·Bioprocess and Biosystems Engineering·Pratibha BaralDeepti Agrawal
Jan 26, 2020·Microorganisms·Raja Mohan GopalakrishnanKlaus Heese
Feb 15, 2020·Journal of the Science of Food and Agriculture·Ana Belén DíazAna Blandino
Feb 23, 2020·Applied Microbiology and Biotechnology·Heeyoung ParkSoo Rin Kim
Jul 20, 2019·Biotechnology for Biofuels·Abhishek S PatriCharles E Wyman
Apr 27, 2018·Bioengineering·Augusto C M SouzaKurt A Rosentrater
Jul 6, 2019·Frontiers in Chemistry·Alex K Chew, Reid C Van Lehn
Oct 19, 2019·Biotechnology for Biofuels·Oscar Rosales-Calderon, Valdeir Arantes
Jan 31, 2020·Biotechnology for Biofuels·Jie WangDominic Sauvageau
Mar 18, 2020·Microbial Cell Factories·Aritha DornauSimon J McQueen-Mason
May 2, 2018·Biotechnology for Biofuels·Lei QinYing-Jin Yuan
Mar 27, 2019·Biotechnology for Biofuels·Xiaolin LuoLi Shuai
Jul 9, 2020·Proceedings of the National Academy of Sciences of the United States of America·Sai Venkatesh PingaliLoukas Petridis
Apr 28, 2020·Biotechnology for Biofuels·Minsheng LuWeihua Xiao
Oct 20, 2018·Bioresource Technology·Xianzhi MengArthur J Ragauskas
Jan 3, 2021·Bioresource Technology·Huisheng LyuZhongfeng Geng
Jan 16, 2021·Chemistry, an Asian Journal·Zhigang Chai
Feb 9, 2021·Bioresource Technology·Ramkrishna SinghVijay Singh
Jan 26, 2020·Bioresource Technology·Chang Geun YooArthur J Ragauskas
May 3, 2019·Carbohydrate Polymers·Roi PeretzHadas Mamane
Jan 14, 2020·Bioresource Technology·Shashi Kant BhatiaYung-Hun Yang
Mar 31, 2021·Applied Biochemistry and Biotechnology·Xutong Ma, Yong Xu
Apr 25, 2021·Biotechnology for Biofuels·Peng NingJianming Yang
May 1, 2021·Polymers·Yuan ZhuYinhua Wan
May 14, 2021·Bioresource Technology·T VancovB Keen
Jun 25, 2021·Nature Communications·Zhi-Hua LiuJoshua S Yuan
Jul 11, 2021·Applied Biochemistry and Biotechnology·Hailong LiXinde Chen
Dec 4, 2019·ACS Omega·Kaneza PascalJing He
Jul 16, 2019·Journal of the American Chemical Society·Abhishek S PatriCharles M Cai
Sep 18, 2021·Critical Reviews in Biotechnology·Pratibha BaralDeepti Agrawal