Overestimation of the 25(OH)D serum concentration with the automated IDS EIA kit

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Etienne CavalierJean-Claude Souberbielle

Abstract

We have recently observed an increasing number of patients presenting very high serum levels of 25-hydroxyvitamin D [25(OH)D] (> 150 ng/mL), which, in all cases, had been measured with the IDS EIA kit adapted on different "open" automated platforms. We performed a comparison between the IDS EIA kit adapted on two different "open"automated platforms and the DiaSorin RIA. We found a systematic bias (higher levels with the IDS EIA kit) for concentrations more than 50-60 ng/mL that was less obvious when the IDS EIA was used in its manual procedure. We thus suggest to use the IDS EIA kit in its manual procedure rather than to adapt it on an automated platform, and to interpret cautiously a 25(OH)D greater than 100 ng/mL with this kit.

References

Mar 19, 2005·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Bess Dawson-HughesReinhold Vieth
Jul 11, 2006·The American Journal of Clinical Nutrition·Heike A Bischoff-FerrariBess Dawson-Hughes
Jul 20, 2007·The New England Journal of Medicine·Michael F Holick
Nov 9, 2007·The New England Journal of Medicine·Roger BouillonPaul Lips
Apr 19, 2008·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·R PrzybelskiN Binkley
Jul 4, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Etienne CavalierJean-Claude Souberbielle
Sep 23, 2008·Annales d'endocrinologie·J-C SouberbielleC Cormier
Jul 23, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Guillaume JeanCharles Chazot
Feb 6, 2010·The Journal of Clinical Endocrinology and Metabolism·John S Adams, Martin Hewison

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Citations

Feb 4, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Etienne CavalierJean-Claude Souberbielle
Sep 18, 2015·Critical Reviews in Clinical Laboratory Sciences·Paul Glendenning, Charles A Inderjeeth
Feb 11, 2012·Clinical Biochemistry·Etienne CavalierJean-Claude Souberbielle
Dec 25, 2019·The Journal of Clinical Endocrinology and Metabolism·Mengdi LuAugusto A Litonjua
Nov 6, 2014·The Medical Journal of Australia·Peter R EbelingMichael G Kimlin

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